Optical images of silk fibroin hydrogels were captured using either digital microscopy (AM7915MZTL, Dino-Lite) or smartphones. Fluorescence images of silk fibroin hydrogels were captured under 300 nm ultraviolet illumination of a transilluminator (FBTI-88, Fisher Scientific). Birefringence images were captured using polarized optical microscopy (Eclipse E200POL, Nikon) equipped with a first-order red retardation plate. The cell-laden hydrogels were imaged in a BZ-X700 microscope (Keyence, IL) for live/dead staining, as well as in a Leica SP8 confocal microscope (Leica Microsystems) for F-actin and the autofluorescence of the silk fibroin. Images were processed using ImageJ (NIH).
Eclipse e200pol
Manufactured by Nikon
Sourced in Japan
The Eclipse E200POL is a polarizing microscope designed for optical and materials analysis. It features a sturdy frame, infinity-corrected optical system, and a range of accessories to support various applications. The core function of the Eclipse E200POL is to provide high-quality, polarized light microscopy for scientific and industrial research purposes.
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2 protocols using eclipse e200pol
1
Silk Fibroin Hydrogel Imaging
2
Spherulite Morphology Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
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A polarizing microscope (POM) was used to observe the crystal morphology of spherulites. POM images were collected under a nitrogen atmosphere using a polarizing microscope (Eclipse E200 POL, Nikon, Japan) equipped with a hot table (LTS420; Linkam scientific instruments, Redhill, UK). The sample was melted at 180 °C, and the crystal morphology was observed at 120 °C. The overall crystallization behaviors of samples were also monitored using digital images.
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