Anti phospho erk t202 y204

The antibodies were purchased as follows: Rabbit polyclonal anti-JNK-1 (S-18), anti-ERK-1 (K-23) and anti-p38 (N-20) antibodies were all purchased from Santa Cruz (Dallas, TX, USA). Anti-phospho-ERK (T202/Y204) and anti-phospho-JNK (T183/Y185) were purchased from Cell Signaling Technology (Hitchin, UK). Anti-phospho-p38 (44684-G) was purchased from Applied Biosystems, Life Technologies (Paisley, UK). HRP- conjugated anti-mouse, and conjugated anti-rabbit antibodies were purchased from Jackson Immunoresearch laboratories (Luton, Beds, UK). Anti-Mouse CD34-FITC conjugated, anti-Mouse CD115 (c-fms)-PE conjugated, and recombinant M-CSF were all purchased from eBioscience (Hatfield, UK). SP600125 JNK inhibitor, SB 203580 p38 inhibitor, and U2106 MEK1,2 inhibitor was purchased from Tocris Bioscience (Bristol, UK). BrdU Cell Proliferation kit was purchased from Cell Signaling (New England Biolabs, Hitchin, UK). SuperScript^® III First-Strand Kit (cDNA synthesis system for RT-PCR), oligonucleotide PCR Primers, SYBR^® Select real-time PCR Master Mix were purchased from Applied Biosystems, Life Technologies (Paisley, UK). RNeasy Mini Kit (RNA extraction kit), and RNase-Free DNase Set were obtained from QIAGEN (Manchester, UK). All other materials used were of the highest commercial grade available and they were obtained from Sigma Aldrich (Poole, Dorset, UK).

Recombinant human TGF-β1 was from PeproTech Inc (Rocky Hill, NJ, USA). Xuesaitong injection (the composition is PNS) was purchased from Harbin Zhenbao Pharmaceutical Co., Ltd. Anti-Fibronectin (15613), anti-E-cadherin (20874), anti-Vimentin (10366), anti-Snail (bs-1371R), and anti-TWIST1 (P106287) were purchased from Proteintech Group Inc. (Campbell Park, Chicago, USA). Antiphospho-p38 (Thr180/Tyr182), anti-p38 (D13E1), antiphospho-JNK (Thr183/Tyr185), anti-JNK (56G8) and antiphospho-Erk (T202/Y204), and anti-Erk (137F5) were purchased from Cell Signaling Technology (Danvers, MA, USA). Inhibitors SB203580 (S1076), PD98059 (S1177), and SP600125 (S1460) were purchased from Selleck Inc (Jin Shan, SH, China).