1 1 diphenyl 2 picrylhydrazyl

Manufactured by HiMedia

Sourced in India

1,1-diphenyl-2-picrylhydrazyl is a stable free radical compound used as a reagent in various analytical and research applications. It is often utilized as a standard for assessing antioxidant activity and free radical scavenging properties of compounds.

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2 protocols using 1 1 diphenyl 2 picrylhydrazyl

Phytochemical Analysis and Antioxidant Assays

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Gallic acid (C₇H₆O₅), FC reagent (C₆H₆O), ABTS (C₁₈H₁₈N₄O₆S₄), Ellman’s reagent (C₁₄H₈N₂O₈S₂), sodium carbonate (Na₂CO₃), 1,1-diphenyl-2-picrylhydrazyl (C₁₈H₁₂N₅O₆), ascorbic acid (C₆H₈O₆), aluminum chloride hexahydrate (AlCl₃·6H₂O), sulfuric acid (H₂SO₄), ferric chloride (FeCl₃), bovine serum albumin, lysogeny broth, ethanol, and nutrient agar were purchased from Hi-Media (Mumbai, India). Acetylcholinesterase and silver nitrate were purchased from Sigma (Roeder mark, Germany). Using a spectrophotometer (Shimadzu UV-1601, Tokyo, Japan), calorimetric analysis was carried out.

Singh M.P., Shabir S., Deopa A.S., Raina S.R., Bantun F., Jalal N.A., Abdel-razik N.E., Jamous Y.F., Alhumaidi M.S., Altammar K.A., Hjazi A., Singh S.K, & Vamanu E. (2022). Synthesis of Green Engineered Silver Nanoparticles through Urtica dioica: An Inhibition of Microbes and Alleviation of Cellular and Organismal Toxicity in Drosophila melanogaster. Antibiotics, 11(12), 1690.

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Antioxidant Activity Evaluation Protocols

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1,1-Diphenyl-2-picrylhydrazyl (DPPH), gallic acid, sodium carbonate, ascorbic acid, potassium dihydrogen phosphate, potassium hydrogen phosphate, potassium ferricyanide, trichloro acetic acid, ferric chloride, 2,2’-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), potassium persulfate, mercuric chloride, potassium iodide, sulfuric acid, hydrochloric acid, α-naphthol, magnesium ribbons, acetic anhydride, sodium hydroxide, and ninhydrin were purchased from HiMedia Laboratories Pvt. Ltd., India. Folin-Ciocalteu's reagent was obtained from S d fine-Chem. Ltd., India. All the solvents were of analytical grade and purchased from Sisco Research Laboratories Pvt. Ltd., India.

Ravikumar P., Shalini G, & Jeyam M. (2015). Wheat Seedlings as Food Supplement to Combat Free Radicals: An In Vitro Approach. Indian Journal of Pharmaceutical Sciences, 77(5), 592-598.

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