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2 protocols using 4 5 o dicaffeoylquinic acid

1

HPLC Analysis of Polyphenol Compounds

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Analytical and HPLC grade solvents and reagents were used for chemical analyses. Acetonitrile (99.9%) and methanol (99.9%)as well as the references as quercetin, quercetin-3-O-glucoside, luteolin-7-O-glucoside, luteolin-7-O-rutinoside, rutin trihydrate, apigenin-7-O-glucoside, santin, 3-O-caffeoylquinic acid, 5-O-caffeoylquinic acid, 4-O-caffeoylquinic acid, 1,3-O-dicaffeoylquinic acid, 3,4-O-dicaffeoylquinic acid, 3,5-O-dicaffeoylquinic acid, 1,5-O-dicaffeoylquinic acid, and 4,5-O-dicaffeoylquinic acid, were obtained from Sigma-Aldrich (Steinheim, Germany); trifluoroacetic acid (≥99%) and apigenin were supplied from Fluka Chemika (Buchs, Switzerland). The purified deionized water (18.2 mΩ/cm) was produced using the Millipore Simpak1 Synergy 185 ultra-pure (Bedford, MA, USA) water system.
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2

Phytochemical Screening of Botanical Extracts

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The chemicals used were analytical reagent grade: ABTZ (ABTZ, Sigma-Aldrich USA), DPPH (DPPH, Sigma-Aldrich USA), Trolox (Sigma-Aldrich USA), Folin-Ciocalteu phenol reagent (Sigma-Aldrich USA), Sodium carbonate (Na 2 CO 3 , Univar Ajax Finechem New Zealand) Aluminium chloride (AlCl 3 , Univar Ajax Finechem New Zealand), Potassium acetate (CH 3 COOK; Univar Ajax Finechem New Zealand), Hydrochloric acid (Lab Scan Thailand), Ethanol (Lab Scan Thailand). HPLC grade: 4,5-O-dicaffeoylquinic acid (Sigma-Aldrich USA), Quercetin (Sigma-Aldrich USA), Gallic acid (Sigma-Aldrich USA), MEthanol (Lab Scan Thailand), Phosphoric acid (Lab Scan Thailand), and Cosmetic grade: Tween 80 and propylene glycol (Namsiang Thailand).
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