Hcc1143

TNBC cell lines BT20, CAL51, HCC70, HCC1143, HCC1187, HCC1806, HCC1937, Hs578T,
MDA-MB-157, MDA-MB-231 and MDA-MB-468 were obtained from the American Tissue
Culture Collection (Rockville, MD, USA). TNBC cell lines CAL120, CAL851 and
HDQP1 were obtained from the German Tissue Repository DMSZ (Braunschweig,
Germany). All cell lines were tested for mycoplasma and authenticated by short
tandem repeat (STR) typing (Additional File 1). The HCC1143, HCC1187, HCC1806,
HCC1937, Hs578T, MDA-MB-231 and MDA-MB-468 cells were cultured in RPMI
(Sigma-Aldrich) containing 10% foetal calf serum (FCS; Life Technologies); the
HCC70 cells were cultured in RPMI containing 10% FCS, 1 mM sodium pyruvate (Life
Technologies) and 2 mM nonessential amino acids (Life Technologies); the HDQP1
cells were cultured in DMEM (Sigma-Aldrich) containing 10% FCS; the CAL51 cells
were cultured in DMEM containing 10% FCS and 1 mM sodium pyruvate; the CAL120
and CAL851 cells were cultured in DMEM (Sigma-Aldrich) containing 10% FCS, 1 mM
sodium pyruvate and 2 mM glutamine (Life Technologies); the BT20 cells were
cultured in DMEM-HAM F12 (Sigma-Aldrich) containing 10% FCS; the MDA-MB-157
cells were cultured in Leibovitz L15 (Sigma-Aldrich) containing 10% FCS. Cells
were incubated at 37°C and 5% CO₂.