The following compounds were used in this study: LPS (cat. L2880, Sigma-Aldrich, USA), GO (cat. W250309, Sigma-Aldrich), IAM (cat. A3221, Sigma-Aldrich), AOAA (cat. C13408, Sigma-Aldrich), and PAG (cat. P7888, Sigma-Aldrich). Antibodies used were IL-6 antibody (cat. 21865-1-AP, Proteintech, China), TNF-α antibody (cat. 60291-1-Ig, Proteintech), IL-1β antibody (cat. 16806-1-AP, Proteintech), IL-18 antibody (cat. 10663-1-AP, Proteintech), p-NFκB p65 antibody (cat. 3033, Cell Signaling Technology, USA), NF-κB p65 antibody (cat. 8242T, Cell Signaling Technology), NLRP3 antibody (cat. 15101S, cat. 8242T, Cell Signaling), ASC antibody (cat. sc-514414, Santa Cruz Biotechnology, USA), cleaved caspase-1 antibody (cat. sc56036, Santa Cruz Biotechnology), GSDMD antibody (cat. A18281, Abclonal, USA), cleaved N-terminal GSDME antibody (cat. ab222407, Abcam, UK), CBS antibody (cat. GTX628777, GeneTex, USA), CSE antibody (cat. 12217-1-AP, Proteintech), 3MST antibody (cat. HPA001240, Sigma-Aldrich), and β-actin antibody (60004-1-Ig, Proteintech).
Il 18 antibody
Manufactured by Proteintech
Sourced in China
The IL-18 antibody is a reagent used in laboratory research to detect and measure the presence of the interleukin-18 (IL-18) protein in biological samples. IL-18 is a pro-inflammatory cytokine involved in immune system regulation. The antibody can be used in various immunoassay techniques, such as ELISA, to quantify IL-18 levels in cell culture supernatants, serum, plasma, and other sample types.
Automatically generated - may contain errors
Lab products found in correlation
Il 1β antibody, by Proteintech (2 mentions)
Nf κb p65 antibody, by Cell Signaling Technology (1 mentions)
Cleaved caspase 1 antibody, by Santa Cruz Biotechnology (1 mentions)
Il 6 antibody, by Proteintech (1 mentions)
Tnf α antibody, by Proteintech (1 mentions)
Asc antibody, by Cell Signaling Technology (1 mentions)
Cse antibody, by Proteintech (1 mentions)
Nlrp3 antibody, by Cell Signaling Technology (1 mentions)
P nf κb p65 antibody, by Cell Signaling Technology (1 mentions)
β actin antibody, by Proteintech (1 mentions)
Ab181440, by Abcam (1 mentions)
Sp 9001 splink detection kits, by OriGene (1 mentions)
Mmp 2 antibody, by Proteintech (1 mentions)
Cd31 antibody, by Abcam (1 mentions)
Gsdmd, by Santa Cruz Biotechnology (1 mentions)
Nanozoomer, by Hamamatsu Photonics (1 mentions)
Fluorescence microscope, by Olympus (1 mentions)
Nlrp3, by Adipogen (1 mentions)
Oil red o, by Solarbio (1 mentions)
3 protocols using il 18 antibody
1
Evaluating Inflammatory Pathways in Cellular Models
2
Immunohistochemical Analysis of Inflammatory Markers
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Paraformaldehyde-fixed paraffin-embedded sections were incubated with primary antibodies overnight at 4°C. The primary antibodies used were against OPN-N (rabbit anti-OPN-N antibody, ab181440, 1:200; Abcam, United Kingdom), NLRP3 (rabbit anti-NLRP3 antibody, 1:200; Boster, Wuhan, China), ASC (rabbit anti-ASC antibody, 10500-1-AP, 1:200; Proteintech, Wuhan, China), IL-18 (rabbit anti- IL-18 antibody, 1:200; Proteintech), IL-1β (rabbit anti-IL-1β antibody, 1:200; Arigo, Hamburg, Germany), Caspase-1 (rabbit anti- Caspase-1 antibody, 22915-1-AP, 1:200; Proteintech), MMP2 (rabbit anti-MMP2 antibody, 1:200; Proteintech), MMP9 (rabbit anti-MMP9 antibody, 1:200; Proteintech), α-smooth muscle actin (SMA) (rabbit anti-α-SMA, 1:200; Proteintech) and α9β1 (rabbit anti-α9β1, ab27947, 1:200; Abcam). As negative controls, species and isotype-matched IgG was applied in place of the primary antibody. Immunohistochemical analysis was performed using the SP-9001 SPlink Detection kits (OriGene Technologies, Inc.), according to the manufacturers’ instructions. All sections were examined using a Nikon E100 microscope. Three sections were randomly selected from each group.
3
Comprehensive Histological Assessment of Atherosclerosis
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Mouse aortas were opened longitudinally from the ascending aorta to the abdominal aorta, and fixed in 4% paraformaldehyde for 36 h. Hematoxylin and eosin (HE) staining assay was used to detect atherosclerotic lesions, and the Oil Red O (Solarbio, China) staining kit was used to evaluate lipid accumulation. Masson staining was used to evaluate the content of collagen fibers in atherosclerotic plaques. Immunofluorescence staining was performed to stain the location of endothelial cells in atherosclerotic lesions using CD31 antibody (1:100, Abcam, USA), and to detect the expression of GSDMD (1:50, Santa Cruz, USA) and the co-localization of endothelial NLRP3 (1:100, AdipoGen, USA) and ASC (1:100, AdipoGen, USA) by visualizing using a fluorescence microscope (Olympus, Japan). Immunohistochemistry staining was performed to stain the level of IL-1β and IL-18 in atherosclerotic lesions using IL-1β antibody (1:100, Proteintech, China) and IL-18 antibody (1:100, Proteintech, China) by visualizing using a Nano Zoomer (Hamamatsu, Japan). Quantitative analysis of lesions was performed using Fiji software (NIH, USA).
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