Total RNA samples were first evaluated for their quantity and quality using Agilent TapeStation. All the samples used for the sequencing had a RIN of at least 5. 100 ng of total RNA were used for library preparation with the KAPA total RNA Hyperprep Kit (KK8581) (Roche). Each resulting uniquely dual-indexed library was quantified and quality accessed by Qubit and Agilent TapeStation. Multiple libraries were pooled in equal molarity. The pooled libraries were sequenced on an Illumina NovaSeq 6000 sequencer with the v1.5 reagent kit. 100 bp paired-end reads were generated.
Kapa total rna hyperprep kit
Manufactured by Roche
The KAPA Total RNA HyperPrep Kit is a laboratory equipment product designed for the preparation of total RNA samples. It offers a streamlined workflow for the isolation and purification of RNA from various sample types. The kit includes reagents and protocols necessary for efficient RNA extraction, concentration, and cleanup, enabling users to obtain high-quality RNA suitable for downstream applications such as gene expression analysis or library preparation.
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Lab products found in correlation
2 protocols using kapa total rna hyperprep kit
1
Total RNA Sequencing Library Preparation
2
Total RNA Sequencing Workflow
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Total RNA samples were first evaluated for their quantity and quality using Agilent TapeStation. All the samples used for the sequencing had a RIN of at least 5. 100 nanograms of total RNA were used for library preparation with the KAPA total RNA Hyperprep Kit (KK8581) (Roche). Each resulting uniquely dual-indexed library was quantified and quality accessed by Qubit and Agilent TapeStation. Multiple libraries were pooled in equal molarity. The pooled libraries were sequenced on an Illumina NovaSeq 6000 sequencer with the v1.5 reagent kit. 100 bp paired-end reads were generated.
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