Acrylamide ≥ 99% (Aam), N,N′-methylenebisacrylamide ≥ 99.5% (Bis), squalane oil, dimethyl sulfoxide (DMSO), 3-(trimethoxysilyl) propyl methacrylate 98% (linker), 1-hydroxycyclohexyl phenyl ketone 99% (HCPK) and 2-amino-2-hydroxymethyl-propane-1,3-diol (Tris) were purchased from Sigma-Aldrich; ethylenediaminetetraacetcic acid (EDTA) and sodium chloride (NaCl) were obtained from VWR. Single-stranded Morpholino oligonucleotides and DNA oligonucleotides with custom specified base pair sequence (Table 1 ) (some functionalized with an acrydite group, some fluorescently labelled at specific base) were obtained from Gene Tools (Philomath, OR, USA) and Integrated DNA Technologies (IDT, Coralville, IA, USA), respectively. All materials were used without further purification. De-ionized water with resistivity 18.2 MΩ cm (Millipore Milli-Q) was used throughout.
3 trimethoxysilyl propyl methacrylate 98
Manufactured by Merck Group
Sourced in United States, Germany
3-(trimethoxysilyl) propyl methacrylate 98% is a silane coupling agent used as a surface modifier in the production of various materials. It functions to improve the bonding and adhesion between organic and inorganic components.
Automatically generated - may contain errors
Lab products found in correlation
2 amino 2 hydroxymethyl propane 1 3 diol, by Merck Group (1 mentions)
Milli q, by Merck Group (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Analytical balance, by Shimadzu (1 mentions)
Consepsis, by Ultradent (1 mentions)
Fibrinogen, by Merck Group (1 mentions)
Milli q plus system, by Merck Group (1 mentions)
Human serum albumin (hsa), by Merck Group (1 mentions)
Tween 20, by Merck Group (1 mentions)
Isoamyl alcohol, by Merck Group (1 mentions)
Acetonitrile, by Thermo Fisher Scientific (1 mentions)
Methanol, by Thermo Fisher Scientific (1 mentions)
1 4 butanediol, by Merck Group (1 mentions)
2 2 azobis 2 methylpropionitrile, by Merck Group (1 mentions)
Stearyl methacrylate, by Merck Group (1 mentions)
Mgcl2, by Merck Group (1 mentions)
Hepes, by Merck Group (1 mentions)
Nahco3, by Merck Group (1 mentions)
Sodium chloride (nacl), by Merck Group (1 mentions)
Potassium chloride (kcl), by Merck Group (1 mentions)
4 protocols using 3 trimethoxysilyl propyl methacrylate 98
1
Hydrogel Biomaterial Synthesis Protocol
2
Silanized Nanotubes for Disinfection
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One gram of HNs (Sigma–Aldrich, St. Louis, MO, USA) that had been previously dried in a HERAtherm drying oven (Thermo Fisher Scientific, Waltham, MA, USA) was weighed using an analytical balance (Shimadzu Scientific Instruments, Kyoto, Japan). A solution of 3-(trimethoxysilyl) propyl-methacrylate-98% (Sigma–Aldrich, St. Louis, MO, USA) diluted to 5% and 95% acetone (Sigma–Aldrich, St. Louis, MO, USA) was used for the immersion of the nanotubes for 24 h at 110 °C in a drying oven.
Subsequently, 1 g of silanized nanotubes was mixed with 10 mL of 2% CX (Consepsis, Ultradent Products, South Jordan, UT, USA), commonly used for disinfection in dentistry [21 (link)], and 10 mL of 95% pure ethanol and sonicated for 1 h. The CX-loaded nanotubes were then placed in a drying oven for 10 days at 30 °C to eliminate residual solvent [17 (link)].
Subsequently, 1 g of silanized nanotubes was mixed with 10 mL of 2% CX (Consepsis, Ultradent Products, South Jordan, UT, USA), commonly used for disinfection in dentistry [21 (link)], and 10 mL of 95% pure ethanol and sonicated for 1 h. The CX-loaded nanotubes were then placed in a drying oven for 10 days at 30 °C to eliminate residual solvent [17 (link)].
3
Fabrication of Bioinspired Adhesive Interfaces
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Gelatin from porcine skin (type A; gel strength
∼175 bloom), phosphate buffered saline tablets (PBS; 0.01 M
phosphate buffer), 3-(trimethoxysilyl)propyl methacrylate 98%, Tween
20, and fibrinogen from human plasma were purchased from Sigma-Aldrich
Chemie GmbH Taufkirchen, Germany. Anhydrous dimethylformamide (DMF)
was obtained from VWR International GmbH Bruchsal, Germany. Human
serum albumin (HSA; fraction V, high purity) and sodium dodecyl sulfate
(SDS) was purchased from Merck KGaA Darmstadt, Germany. Hellmanex
II solution (1 vol % solution in water) was bought from Hellma, Müllheim,
Germany.l -Lysin diisocyanate ethyl ester (LDI) was obtained
from Yipeng Chemical Co., China, and was distilled prior to use. Ultrapure
water from a Milli-Q plus system (Millipore, Schwalbach, Germany)
was used for the preparation of buffer, protein, and gelatin solutions.
Silicon wafers were obtained from Silicon Materials, Kaufering, Germany.
When used as Si substrates, wafers were first silanized with 3-(trimethoxysilyl)propyl
methacrylate. When used to produce gold wafers, they were sputtered
with 5 nm titanium and 100 nm gold. QCM-D sensor crystals with top
gold electrode (gold-coated AT-cut quartz crystals, QSX301, 5 MHz)
were purchased from Q-Sense AB, Göteborg, Sweden.
∼175 bloom), phosphate buffered saline tablets (PBS; 0.01 M
phosphate buffer), 3-(trimethoxysilyl)propyl methacrylate 98%, Tween
20, and fibrinogen from human plasma were purchased from Sigma-Aldrich
Chemie GmbH Taufkirchen, Germany. Anhydrous dimethylformamide (DMF)
was obtained from VWR International GmbH Bruchsal, Germany. Human
serum albumin (HSA; fraction V, high purity) and sodium dodecyl sulfate
(SDS) was purchased from Merck KGaA Darmstadt, Germany. Hellmanex
II solution (1 vol % solution in water) was bought from Hellma, Müllheim,
Germany.
from Yipeng Chemical Co., China, and was distilled prior to use. Ultrapure
water from a Milli-Q plus system (Millipore, Schwalbach, Germany)
was used for the preparation of buffer, protein, and gelatin solutions.
Silicon wafers were obtained from Silicon Materials, Kaufering, Germany.
When used as Si substrates, wafers were first silanized with 3-(trimethoxysilyl)propyl
methacrylate. When used to produce gold wafers, they were sputtered
with 5 nm titanium and 100 nm gold. QCM-D sensor crystals with top
gold electrode (gold-coated AT-cut quartz crystals, QSX301, 5 MHz)
were purchased from Q-Sense AB, Göteborg, Sweden.
4
Artificial Seawater Composition for Marine Research
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Artificial seawater (ASW) contained 460 mM NaCl, 26 mM MgSO4, 22 mM MgCl2, 15 mM HEPES, 10 mM KCl, 10 mM CaCl2, and 2.5 mM NaHCO3, all purchased from Sigma-Aldrich (St. Louis, MO). The solution was adjusted to pH 7.8 and supplemented with antibiotics. Methanol, acetonitrile (ACN), formic acid (FA), and ethylene glycol dimethacrylate 98% (EDMA) were purchased from Fisher Scientific (Fairlawn, NJ). Stearyl methacrylate (SMA), 3-(trimethoxysilyl)propyl methacrylate 98% (TMSPM), iso-amyl alcohol (IAA), 2,2′-azobis(2-methylpropionitrile) (AIBN), and 1,4-butanediol (BD) were obtained from Sigma-Aldrich.
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