Magnesium 1m

Manufactured by Thermo Fisher Scientific

Magnesium 1M is a laboratory reagent used as a source of magnesium ions. It is a clear, colorless solution of magnesium chloride in water, with a concentration of 1 mol/L (1M). Magnesium 1M is commonly used in various analytical and experimental procedures that require the availability of magnesium ions.

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Lab products found in correlation

6 hydroxy 2 5 7 8 tetramethylchromane 2 carboxylic acid trolox, by Merck Group (2 mentions) Bsa biotin, by Merck Group (2 mentions) Methanol, by Merck Group (2 mentions) Protocatechuate 3 4 dioxygenase pseudomonas pcd, by Merck Group (2 mentions) Peg 8000, by Merck Group (1 mentions) Glass slide, by Thermo Fisher Scientific (1 mentions) Glycerol, by Merck Group (1 mentions) Tween 20, by Merck Group (1 mentions) Sodium chloride 5m, by Thermo Fisher Scientific (1 mentions) 3 4 dihydroxybenzoic acid pca, by Merck Group (1 mentions) Glass slide, by Avantor (1 mentions) Ultrapure water, by Thermo Fisher Scientific (1 mentions) Unmodified dna oligonucleotides, by Eurofins (1 mentions)

2 protocols using magnesium 1m

DNA Oligonucleotide-based Microscopy Assay

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Unmodified, dye-labeled, and biotinylated DNA oligonucleotides were purchased from MWG Eurofins. DNA scaffold strands were purchased from Tilibit (p7249, identical to M13mp18). Streptavidin (cat: S-888) and glass slides (cat: 10756991) were ordered from Thermo Fisher. Coverslips were purchased from Marienfeld (cat: 0107052). PEG-8000 was purchased from Merck (cat: 6510-1KG). Tris 1M pH 8.0 (cat: AM9856), EDTA 0.5M pH 8.0 (cat: AM9261), Magnesium 1M (cat: AM9530G) and Sodium Chloride 5M (cat: AM9759) were ordered from Ambion. Ultrapure water was obtained from a Milli-Q filter machine. Tween-20 (cat: P9416-50ML), Glycerol (cat: G5516-500ML), Methanol (cat: 32213-2.5L), BSA-Biotin (cat: A8549), Protocatechuate 3,4-Dioxygenase Pseudomonas (PCD) (cat: P8279), 3,4-Dihydroxybenzoic acid (PCA) (cat: 37580-25G-F) and (+-)-6-Hydroxy-2,5,7,8-tetra-methylchromane-2-carboxylic acid (Trolox) (cat: 238813-5G) were ordered from Sigma-Aldrich. Twinsil two-component glue was purchased from Picodent (cat: 13001000). Monoclonal antibodies against alpha-tubulin (cat: MA1-80017) was purchased from Thermo Scientific. The secondary antibodies Anti-Rat (cat: 712-005-150) were purchased from Jackson ImmunoResearch.

Stehr F., Stein J., Schueder F., Schwille P, & Jungmann R. (2019). Flat-top TIRF illumination boosts DNA-PAINT imaging and quantification. Nature Communications, 10, 1268.

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Single-Molecule DNA Origami Assembly

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Unmodified DNA oligonucleotides, fluorescently modified DNA oligonucleotides, and biotinylated DNA oligonucleotides were purchased from MWG Eurofins. M13mp18 scaffold was obtained from Tilibit. BSA-Biotin was obtained from Sigma-Aldrich (cat: A8549). Streptavidin was ordered from Invitrogen (cat: S-888). Tris 1 M pH 8.0 (cat: AM9856), EDTA 0.5 M pH 8.0 (cat: AM9261), Magnesium 1 M (cat: AM9530G) and Sodium Chloride 5 M (cat: AM9759) were ordered from Ambion. Ultrapure water (cat: 10977-035) was purchased from Gibco. Polyethylene glycol (PEG)-8000 (catalog no. 6510-1KG) was purchased from Merck. Tween-20 (P9416-50ML), glycerol (65516-500 ml), methanol (32213-2.5 L), protocatechuate 3,4-dioxygenase pseudomonas (PCD; P8279), 3,4-dihydroxybenzoic acid (PCA; 37580-25G-F) and (±)-6-hydroxy- 2,5,7,8-tetra-methylchromane-2-carboxylic acid (trolox; 238813-5 G) were ordered from Sigma-Aldrich. Glass slides (cat: 48811-703) were obtained from VWR. Coverslips were purchased from Marienfeld (cat: 0107032). Silicon (cat.1300 1000) was ordered from picodent. Double-sided tape (cat: 665D) was ordered from Scotch.

Huijben T.A., Heydarian H., Auer A., Schueder F., Jungmann R., Stallinga S, & Rieger B. (2021). Detecting structural heterogeneity in single-molecule localization microscopy data. Nature Communications, 12, 3791.

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