Mouse anti human cd206 monoclonal antibody

Lung biopsy specimens were obtained from autopsy cases, in which the patient died of severe pneumonia, and resected lung from a patient with early lung cancer was also examined. Tissues were fixed in 10% formalin and embedded in paraffin. Deparaffinized sections (5-μm-thick) were immersed in epitope retrieval solution (Target Retrieval Solution S1700; Dako North America, Inc., Carpinteria, CA, USA) and preheated at 120 °C for 10 min. After blocking endogenous peroxidase activity with 3% H₂O₂ for 15 min, slides were incubated overnight with a mouse anti-human CD206 monoclonal antibody (15 µg/ml; R&D Systems, Minneapolis, MN, USA) or IgG2b at 4 °C. Subsequently, sections were incubated with visualization reagent (ChemMate Envision kit; Dako Japan, Inc., Tokyo, Japan) for 30 min, followed by counterstaining with hematoxylin.

Lung and pleural specimens were obtained from one patient with early lung cancer and PTB by partial resection and three patients with TB pleurisy by using thoracoscopy under local anaesthesia. Tissues were fixed in 10% formalin and embedded in paraffin. Deparaffinized sections (5-μm thick) were immersed in epitope retrieval solution (Target Retrieval Solution S1700; Dako North America, Inc., Carpinteria, CA, USA) and preheated at 120 °C for 10 min. After blocking endogenous peroxidase activity with 3% H₂O₂ for 15 min, slides were incubated overnight with a mouse anti-human CD206 monoclonal antibody (15 µg/ml; R&D Systems, Minneapolis, MN, USA) or IgG2b at 4 °C. Subsequently, sections were incubated with visualization reagent (ChemMate Envision kit; Dako Japan, Inc., Tokyo, Japan) for 30 min, followed by counterstaining with haematoxylin.