Dmpe peg2000

Manufactured by NOF corporation

DMPE-PEG2000 is a polyethylene glycol (PEG) derivative used as a surfactant in various laboratory applications. It is composed of 1,2-Distearoyl-sn-glycero-3-phosphoethanolamine (DMPE) conjugated to a PEG molecule with an average molecular weight of 2000 Daltons. DMPE-PEG2000 is commonly used to stabilize and solubilize various compounds in aqueous solutions.

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Lab products found in correlation

Formaldehyde, by Merck Group (2 mentions) Cholesterol, by Merck Group (2 mentions) Digitonin, by Merck Group (2 mentions) Pioglitazone, by AstraZeneca (2 mentions) Transferrin alexa fluor 568, by Thermo Fisher Scientific (1 mentions) Egf alexa fluor 488, by Thermo Fisher Scientific (1 mentions) Insulin, by AstraZeneca (1 mentions) Streptomycin, by Thermo Fisher Scientific (1 mentions) Penicillin, by Thermo Fisher Scientific (1 mentions) Saponin, by Merck Group (1 mentions) Dexamethasone (dex), by Merck Group (1 mentions) Triton x 100, by Serva Electrophoresis (1 mentions) Fbs superior, by Merck Group (1 mentions) 3 isobutyl 1 methyxanthine, by Merck Group (1 mentions)

2 protocols using dmpe peg2000

Liposome Delivery of Fluorescent-Labeled Biomolecules

Cited 1 time

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FBS Superior, dexamethasone, 3-isobutyl-1-methyxanthine, insulin, and pioglitazone were provided by Astra Zeneca. P/S, transferrin Alexa Fluor 568 (T23365; Invitrogen), EGF Alexa Fluor 488 (E13345; Invitrogen), LDL-pHrodo Red (L34356; Invitrogen), LDL-Alexa Fluor 488 (homemade) were as previously reported (Franke et al., 2019 (link)). pH calibration buffers were from Invitrogen (P35379). LNPs were prepared using the cationic ionizable lipids MC3, L608, ACU5, ACU22, MOD5, and L319 (AstraZeneca) and the helper lipids cholesterol (Sigma-Aldrich), 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC; CordenPharma), 1,2-dimyristoyl-sn-glycero-3-phosphoethanolamine-N [methoxy (polyethylene glycol)-2000] (DMPE-PEG2000; NOF Corporation), and contained CleanCap eGFP mRNA (5-methoxyuridine, cat. no. L-7201) and/or CleanCap Cy5 eGFP mRNA (5-methoxyuridine, batch no. WOTL18871, cat. no. L-7701; TriLink Bio Technologies). Formaldehyde was from Merck and digitonin from Sigma-Aldrich.

Paramasivam P., Franke C., Stöter M., Höijer A., Bartesaghi S., Sabirsh A., Lindfors L., Arteta M.Y., Dahlén A., Bak A., Andersson S., Kalaidzidis Y., Bickle M, & Zerial M. (2021). Endosomal escape of delivered mRNA from endosomal recycling tubules visualized at the nanoscale. The Journal of Cell Biology, 221(2), e202110137.

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Formulation and Characterization of Lipid Nanoparticles

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Formaldehyde (Merck), Triton X-100 (SERVA Electrophoresis GmbH), Digitonin (Sigma Aldrich) and Saponin (Sigma Aldrich), l-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) (Pierce 22980), Disuccinimidyl suberate (DSS) (Pierce 21555), FBS Superior (Merck S0615), dexamethasone (Sigma Aldrich, D2915), 3-isobutyl-1-methyxanthine (Sigma Aldrich I5879), Insulin (Actrapid Novo Nordisk) and Pioglitazone (AZ10080838) were provided by AstraZeneca. Penicillin and streptomycin (Gibco 15140-122). LNPs were prepared with ((2-(dimethylamino)ethyl)azanediyl)bis(hexane-6,1-diyl)bis(2-hexyldecanoate) (AstraZeneca), cholesterol (Sigma Aldrich), 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC; CordenPharma), dn 1,2-dimyristoyl-sn-glycero-3-phosphoethanolamine-N [methoxy (polyethyleneglycol)-2000] (DMPE-PEG2000; NOF Corporation) and contained CleanCap enhanced green fluorescent protein (eGFP) mRNA (5-methoxyuridine) (TriLink Biotechnologies L-7201) and/or Clean CapCyanine5 (Cy5) enhanced green fluorescent protein mRNA (5-methoxyuridine) (TriLink Biotechnologies L-7701).

Paramasivam P., Stöter M., Corradi E., Dalla Costa I., Höijer A., Bartesaghi S., Sabirsh A., Lindfors L., Yanez Arteta M., Nordberg P., Andersson S., Baudet M.L., Bickle M, & Zerial M. (2022). Quantitative intracellular retention of delivered RNAs through optimized cell fixation and immunostaining. RNA, 28(3), 433-446.

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