Activated protective antigen

Manufactured by List Biological Laboratories

Sourced in United States

Activated protective antigen is a key component used in the production of some vaccines. It functions as an immunogen, stimulating the immune system to generate protective antibodies against specific pathogens.

Automatically generated - may contain errors

Lab products found in correlation

Pqe30, by Qiagen (1 mentions) Victor x4 multilabel plate reader, by PerkinElmer (1 mentions)

2 protocols using activated protective antigen

Bacterial Strain Expression Optimization

Check if the same lab product or an alternative is used in the 5 most similar protocols

The bacterial strains used in this study are listed in Table 1. The expression vector pPROEXHTa was purchased from Invitogen (USA), pQE30 from Qiagen (Germany), and pET32c from Novagen (Germany). Luria-Bertani broth/agar for maintaining the expression hosts was obtained from Himedia (India). Activated protective antigen was procured form List Biological Laboratories (USA). All other chemicals were procured from Sigma Aldrich (India) unless specified.

Suryanarayana N., V.a.n.l.a.l.h.m.u.a.k.a., Mankere B., Verma M., Thavachelvam K, & Tuteja U. (2016). Soluble Expression and Characterization of Biologically Active Bacillus anthracis Protective Antigen in Escherichia coli. Molecular Biology International, 2016, 4732791.

+ Open protocol

+ Expand

Anthrax Immune Globulin Preparation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Purified recombinant native protective antigen [83 kDa (1 μM=83μg/ml); PA83] was obtained from BEI Resources (Manassas, VA). Activated protective antigen [63 kDa (1 μM=63μg/ml); PA63] and recombinant lethal factor [90 kDa (1 μM=90 μg/ml); LF] were obtained from List Biological Laboratories (Campbell, CA). Dissociation-enhanced lanthanide fluorescent immunoassay (DELFIA) buffer, wash concentrate, enhancement solution, Streptavidin Microtitration 96-well plates, Platewash, Plateshake, and Victor™ X4 Multilabel Plate Reader were from Perkin-Elmer Life Sciences (Shelton, CT). Lethal toxin (LTx) was prepared by combining PA63 and LF in a 7:4 ratio. Anthrax Immune Globulin (AIG), an investigational product for anthrax treatment consisting primarily of anti-PA antibody, was previously acquired from Cangene (Winnipeg, MB, Canada).

Stoddard R.A., Quinn C.P., Schiffer J.M., Boyer A.E., Goldstein J., Bagarozzi D.A., Soroka S.D., Dauphin L.A, & Hoffmaster A.R. (2014). Detection of anthrax protective antigen (PA) using europium labeled anti-PA monoclonal antibody and time-resolved fluorescence. Journal of immunological methods, 408, 78-88.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!