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3 protocols using monosodium phosphate

1

TPU Nanoparticle Preparation and Antimicrobial Evaluation

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TPU particles were obtained from the Shanghai Yuanneng Plastic Co., Ltd. (Shanghai China). AgNO3 (>99.8%), ammonia (25–28%), sodium borohydride (≥97%), sodium chloride (99.5%), monosodium phosphate (99%), WPU (32%), DMF (≥99.5%), and acetone (≥99.5%) were obtained from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Gram-negative E. coli (ATCC 25922) and Gram-positive S. aureus (CMCC 26003) were obtained from the Shanghai Luwei Technology Co., Ltd. (Shanghai, China). Nutrient agar and broth were obtained from the Hangzhou Baisi Biology Technology Co., Ltd. (Hangzhou, China).
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2

Pomelo Fruitlets Chemical Analysis

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Pomelo fruitlets were obtained from Zhangzhou Pomelo Township Food Co., Ltd. (Zhangzhou, China). Monosodium phosphate, sodium lauryl sulfate, ethylenediaminetetraacetic acid, concentrated hydrochloric acid (HCl), concentrated sulfuric acid (H2SO4), sodium hydroxide (NaOH), hydrogen peroxide (H2O2), and anhydrous magnesium sulfate were of analytical grade, and bought from Sinopharm Chemical Reagent Co. Ltd. (Shanghai, China).
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3

Antioxidant and Tyrosinase Inhibition

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Iodized salt (food grade) was obtained from China National Salt Industry Corporation (Shanghai, China). 2,2-Diphenyl-1-picrylhydrazyl (DPPH), 3,4-dihydroxy-l-phenylalanine (l-DOPA), and tyrosinase were purchased from Sigma-Aldrich (Shanghai, China). Diethyl ether, disodium hydrogen phosphate, monosodium phosphate, anhydrous ethyl alcohol, dimethyl sulfoxide, potassium bichromate, potassium iodide, chloroform, and acetic acid were purchased from Sinopharm (Shanghai, China). Instant cornstarch was obtained from Shanghai Rongshi (Shanghai, China). 2,6-Di-tert-butyl-4-methylphenol (BHT) and ascorbic acid (vitamin C) were purchased from Yingyuan (Shanghai, China) and Fine Chemicals Institute (Shanghai, China), respectively. All commercial essential oils were purchased from Pan'an Tianlan fragrance Co., Ltd (Zhejiang, China), manufactured via steam distillation. All commercial chemicals were used without further purification.
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