Antibody against β actin a1978

Antibodies against protein resistin (sc‐376336), TLR4 (sc‐293072), NF‐κB (sc‐372), PI3K p‐p85 (sc‐12929R), EGFR (sc‐377229), and phosphorylated (p‐)EGFR (sc‐12351) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Antibodies against Twist1 (WL0109), PI3K p85 (WL01169), Akt (WL0003b), c‐Src (WL01570), p‐Src (WL02114), and p‐Akt (WL03307) were purchased from Wanleibio (Shenyang, China). Antibodies against MMP2 (#4022S) and GST (#2622) were purchased from Cell Signaling Technology (Beverly, MA, USA). Antibody against β‐actin (A1978) was purchased from Sigma (St. Louis, MO, USA). Horseradish peroxidase‐conjugated goat anti‐rabbit IgG (AP132F) and goat anti‐mouse (401215) secondary antibodies were bought from Sigma. Recombinant human resistin and resistin with His‐tag were purchased from ProSpec (Rehovot, Israel). Agarose bead‐conjugated anti‐His mouse clonal antibody (AT0084) was purchased from CMCTAG (San Diego, CA, USA). SB203580 (p38 MAPK inhibitor) and LY294002 (PI3K inhibitor) were purchased from Beyotime (Jiangsu, China). Diphenyliodonium (reactive oxygen species [ROS] inhibitor) was purchased from Sigma. Erlotinib HCl (EGFR inhibitor) was purchased from Selleck Chemicals (Houston, TX, USA). TAK242 (TLR4 inhibitor) was purchased from MedChem Express (Monmouth Junction, NJ, USA).

d-limonene was purchased from Sigma-Aldrich (St Louis, MO, USA). Antibody against β-actin (A1978) was purchased from Sigma-Aldrich, and antibodies against BAX (32503), cleaved PARP (32064), LC3B (ab48394), and Atg5 (ab108327) were purchased from Abcam (Cambridge, UK). Small-interfering RNA (siRNA) against atg5 and the mRFP-GFP-LC3 plasmid were obtained from GenePharma (Shanghai, China). The BD FITC Annexin V Apoptosis Detection Kit I was obtained from BD Biosciences (San Jose, CA, USA). The Cell Counting Kit-8 was purchased from Dojindo Laboratories (Kumamoto, Japan). Matrigel^® was purchased from Corning (Coring, NY, USA). BALB/c mice were obtained from Shanghai SLAC Laboratory Animal Co. (Shanghai, China). Rhodamine 123 was purchased from Beyotime (Shanghai, China).