Sandwich elisa

Manufactured by USCN

Sourced in United States, China

The Sandwich ELISA is a type of enzyme-linked immunosorbent assay (ELISA) used for the detection and quantification of target analytes in a sample. It utilizes two antibodies that bind to different epitopes of the target analyte, capturing it between the two antibodies. This method enables the specific and sensitive detection of the target analyte in a sample.

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Lab products found in correlation

Dxc800, by Beckman Coulter (1 mentions)

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Sandwich ELISA kit (10 citations) ELISAs (7 citations) Double-antibody sandwich ELISA kit (3 citations)

4 protocols using sandwich elisa

Ataxin-10 Serum Levels in Pancreatic Cancer Cachexia

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Human serum samples and clinical data of patients with pathologically confirmed pancreatic ductal adenocarcinoma were derived from the prospective biobank and database of the European Pancreas Center (EPC), Department of General Surgery, Heidelberg University Hospital and were collected as approved by the local ethics committee.
Ataxin-10 (Atxn10) serum levels were determined in pre-surgery serum samples by using pre-coated Sandwich-ELISA (Uscn Life Science, Wuhan, CA, USA). Atxn10 serum levels were compared between control groups of patients with pancreatic ductal adenocarcinoma (PDAC) without cachexia (defined according to consensus classification from 2011 [25] (link)), which did or did not receive neoadjuvant radio- and/or chemotherapy (referred to as neoTx) and groups of PDAC patients with cachexia, which did or did not receive neoTx. Clinico-pathological data include tumor characteristics, therapy and weight loss and are provided in Table 3.

Schäfer M., Oeing C.U., Rohm M., Baysal-Temel E., Lehmann L.H., Bauer R., Volz H.C., Boutros M., Sohn D., Sticht C., Gretz N., Eichelbaum K., Werner T., Hirt M.N., Eschenhagen T., Müller-Decker K., Strobel O., Hackert T., Krijgsveld J., Katus H.A., Berriel Diaz M., Backs J, & Herzig S. (2015). Ataxin-10 is part of a cachexokine cocktail triggering cardiac metabolic dysfunction in cancer cachexia. Molecular Metabolism, 5(2), 67-78.

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Evaluating Kidney Injury Biomarkers in SYNERGY

Cited 2 times

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SYNERGY will include kidney damage as a secondary outcome using 24 hr urine samples (gold standard) for measuring proteinuria and albuminuria [45 (link)]. Participants will be provided with bottles for collection and educated on correct collection procedures in accordance with standard protocol. Urinary total protein (pyrogallol red) and urinary albumin (turbidimetric method based on antibody-antigen complexes) will be measured in timed-samples (mg/24 hrs) and cross-checked against creatinine ratios (protein and albumin, respectively) on Beckman DxC800 general chemistry analyser (Beckman Coulter, Brea, CA, USA) [46 ].
Kidney function using the Chronic Kidney Disease Epidemiology Collaboration (CKD-EPI) formula will also be measured [22 ].
In addition, urinary Kim-1, a marker of kidney tubule damage, will be measured from midstream urine collections before and after each intervention [47 (link)]. Samples will be stored at −80°C, followed by analysis using a commercially available sandwich ELISA according to the manufacturer’s instructions (USCN Life Sciences, Wuhan, PRC). Significant changes in Kim-1 have been achieved in a 6 week dietary intervention study indicating the feasibility of Kim-1 as a sensitive marker in SYNERGY [48 (link)].

Rossi M., Johnson D.W., Morrison M., Pascoe E., Coombes J.S., Forbes J.M., McWhinney B.C., Ungerer J.P., Dimeski G, & Campbell K.L. (2014). SYNbiotics Easing Renal failure by improving Gut microbiologY (SYNERGY): a protocol of placebo-controlled randomised cross-over trial. BMC Nephrology, 15, 106.

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Quantification of Urinary Proteins

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Urinary protein candidates were quantitatively analyzed using sandwich ELISA (USCN Life Science Inc., Wuhan, China). Briefly, 100 μL/well of urine samples or standards was added to microtiter plates coated with the specific protein; then, the biotin-conjugated antibodies specific to a protein candidate was added as secondary antibodies for detection. The assay was performed according to the manufacturer’s instructions. The optical density was measured at 405 nm, and the concentration of protein candidates in the samples was calculated based on the standard curve.

Liao W.L., Chang C.T., Chen C.C., Lee W.J., Lin S.Y., Liao H.Y., Wu C.M., Chang Y.W., Chen C.J, & Tsai F.J. (2018). Urinary Proteomics for the Early Diagnosis of Diabetic Nephropathy in Taiwanese Patients. Journal of Clinical Medicine, 7(12), 483.

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Measurement of Retinal RS1 Expression

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Retinal lysates were analyzed with ELISA to detect RS1 expression following the previously described protocol [16 (link),26 (link)]. Briefly, isolated neurosensory retinas from mouse eyes were collected and homogenized with a 25G needle using 200 μl cold PBS. Homogenates were then centrifuged for 10 min at 13,000 ×g, and the protein concentration was then determined with the Bradford (Bio-Rad Protein Assay) method in the cleared supernatants. RS1 concentrations were measured using a sandwich ELISA (USCN Life Science Inc.) according to the manufacturer’s instruction [14 (link)] and visualized as described for MSCs. All samples were analyzed in duplicate, and the results were calculated as pg/100 mg. For all data, the sample size was three.

Bashar A.E., Metcalfe A.L., Viringipurampeer I.A., Yanai A., Gregory-Evans C.Y, & Gregory-Evans K. (2016). An ex vivo gene therapy approach in X-linked retinoschisis. Molecular Vision, 22, 718-733.

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