All cells were plated in no. 1.5 glass-bottomed dishes (MatTek). MEFs were incubated with 0.1 μg/ml Mitotracker Red CMX Ros for 15 min at 37°C with 5% CO2, then washed and incubated with complete media for at least 45 min before imaging. A Z-series with a step size of 0.3 μm was collected with a Nikon Ti-E wide-field microscope with a 63× NA (numerical aperture) 1.4 oil objective (Nikon), a solid-state light source (Spectra X; Lumencor), and an sCMOS camera (Zyla 5.5 megapixel). Each cell line was imaged on at least three separate occasions (n > 100 cells per experiment).
1.4 oil objective
Manufactured by Nikon
The Nikon 1.4 oil objective is a high-performance objective lens designed for microscopy applications. It provides a numerical aperture of 1.4, which enables high-resolution imaging and excellent light-gathering capabilities. The lens is optimized for use with immersion oil, which enhances optical performance and imaging quality. This objective is suitable for a variety of microscopy techniques, including brightfield, phase contrast, and fluorescence microscopy.
Automatically generated - may contain errors
Lab products found in correlation
No 1.5 glass bottomed dishes, by MatTek (2 mentions)
Spectra x, by Lumencor (2 mentions)
Tetracycline hydrochloride, by Thermo Fisher Scientific (1 mentions)
Ti e widefield microscope, by Nikon (1 mentions)
Nucblue, by Thermo Fisher Scientific (1 mentions)
Mitotracker red cmxros, by Thermo Fisher Scientific (1 mentions)
2 protocols using 1.4 oil objective
1
Mitochondrial Imaging in Mouse Embryonic Fibroblasts
2
Mitochondrial Imaging with MitoTracker and NucBlue
Check if the same lab product or an alternative is used in the 5 most similar protocols
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All cells were plated ingrown on no. 1.5 glass-bottomed dishes (MatTek) for ∼48 h before imaging. Cells were incubated with 0.1 μg/ml MitoTracker Red CMXRos with or without three drops NucBlue (Molecular Probes) for 15 min at 37°C with 5% CO2. After this, MEF cells were rinsed into complete media for at least 45 min before imaging, and the Flp-In TREx cells were incubated with 0.2 μg/ml tetracycline hydrochloride (Thermo Fisher Scientific) for 4 h. A Z-series with a step size of 0.3 μm was collected with a Nikon Ti-E wide-field microscope with a 60 × NA (numerical aperture) 1.4 oil objective (Nikon), a solid-state light source (Spectra X; Lumencor), and an sCMOS camera (Zyla 5.5 megapixel). Each cell line was imaged on at least three separate occasions by a blinded experimenter (n > 100 cells per experiment).
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