Poly (D,L-lactide-co-glycolide) (PLGA) (50:50 Resomer RG 502H inherent viscosity 0.16–0.24 dL/g) was purchased from Boehringer Ingelheim (Ingelheim, Germany). Polyethyleneimine (PEI, MW = 10–25 kDa branched), poloxamer 188 (Pluronic® F68), Bradford reagent, potassium phosphate dibasic and potassium phosphate monobasic, sodium azide, sodium chloride, copper (II) sulphate and albumin from chicken egg white (OVA), were purchased from Sigma-Aldrich. Sodium hydroxide was provided from Delchimica Scientific Glassware. Ethanol (96 %), phosphoric acid (85 %), acetic acid, acetonitrile and tetrahydrofuran were purchased from Carlo Erba Reagenti (Milan, Italy).
Potassium phosphate dibasic and potassium phosphate monobasic
Manufactured by Merck Group
Sourced in Italy
Potassium phosphate dibasic and potassium phosphate monobasic are laboratory chemicals used as buffer components. Potassium phosphate dibasic is a white crystalline solid, and potassium phosphate monobasic is a white crystalline powder. These compounds are commonly used in the preparation of buffer solutions to maintain a specific pH range in various laboratory applications.
Automatically generated - may contain errors
Lab products found in correlation
Sodium chloride (nacl), by Merck Group (3 mentions)
Sodium azide, by Merck Group (3 mentions)
Sodium phosphate dibasic, by Merck Group (2 mentions)
Albumin from chicken egg white, by Merck Group (1 mentions)
Copper 2 sulphate, by Merck Group (1 mentions)
Resomer rg502h, by Boehringer Ingelheim (1 mentions)
Acetonitrile, by Carlo Erba (1 mentions)
Ethanol, by Carlo Erba (1 mentions)
Tetrahydrofuran, by Carlo Erba (1 mentions)
Phosphoric acid, by Carlo Erba (1 mentions)
Acetic acid, by Carlo Erba (1 mentions)
Human plasma, by Merck Group (1 mentions)
Human serum albumin (hsa), by Merck Group (1 mentions)
Poloxamer 188 pluronic f 68, by Merck Group (1 mentions)
Pierce quantitative colorimetric peptide assay kit, by Thermo Fisher Scientific (1 mentions)
Methanol meoh, by Merck Group (1 mentions)
1 1 dioctadecyl 3 3 3 3 tetramethylindocarbocyanine perchlorate dii, by Thermo Fisher Scientific (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Merck Group (1 mentions)
Potassium chloride (kcl), by Merck Group (1 mentions)
Deuterated solvent, by Merck Group (1 mentions)
3 protocols using potassium phosphate dibasic and potassium phosphate monobasic
1
PLGA-based Nanoparticle Synthesis Protocol
2
Docetaxel-Loaded Polymeric Nanoparticles
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2.1 Materials Sodium Chloride, DAPI, poloxamer 188 (Pluronic F68) and methanol (MeOH) were from Sigma-Aldrich, Milan, Italy. 1,1′-Dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate (DiI) and the Pierce™ Quantitative Colorimetric Peptide Assay kit were from Thermo Fisher Scientific, Monza, Italy. Dichloromethane (DCM) and acetone were from Honeywells, Monza, Italy. Docetaxel (DTX) was provided by Alpha Aesar. Potassium phosphate dibasic and potassium phosphate monobasic, sodium azide, potassium chloride, sodium phosphate dibasic, human serum albumin (HSA) and human plasma were used as received (Sigma-Aldrich, Italy). Dialysis membranes (MWCO 3500, regenerated cellulose) were used as received (Spectrapor, Italy). The aminoterminated H2N-PCL4.7K-NH2 (daPCL) was synthesized and characterized as previously reported (Conte et al., 2019a) .
3
Synthesis and Characterization of Polyplexes
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All solvents were of analytical or HPLC grade and purchased from Sigma or Fisher Scientific unless otherwise stated. Deuterated solvents were from Sigma. Acryloyl chloride, ethylene-dioxy-bisethylamine, triethylamine (TEA), dithiodiethanol, sodium chloride, potassium phosphate dibasic and potassium phosphate monobasic, sodium azide, sodium phosphate dibasic, ethidium bromide (EtBr), fluorescamine, polyethylenimine (PEI) (25 kDa, branched) calf thymus DNA and glutathione (GSH), were used as received from Sigma Aldrich. Luciferase siRNA (CCGCAAGAUCCGCGAGAUU) was provided by Eurogentec (UK). Luciferase Assay System with Reporter Lysis Buffer and CellTiter 96 s AQueous One Solution Cell Proliferation Assay (MTS) were provided by Promega (UK). Silencert Cyt3-labeled Negative Control was provided by Thermofisher Scientific (UK).
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