Cary 3500 uv vis multicell peltier spectrophotometer

An Agilent (Melbourne, VIC, Australia) Technologies Cary 3500 UV-Vis Multicell Peltier spectrophotometer was utilised to perform the UV spectroscopy experiments. UV spectroscopic experiments were completed at room temperature in the range of 200–400 nm with a 1 cm quartz cuvette. All complexes were prepared in d.i.H₂O. A stock solution of each complex (1 mM) was prepared and absorption spectra were recorded at a series of different concentrations by titrating 9 $\times$ 3 µL aliquots into a cuvette containing d.i.H₂O (3000 µL). Experiments were repeated in triplicate. All spectra were baseline-corrected by the instrument—a baseline containing d.i.H₂O was acquired first, and automatically subtracted from each experiment. Average extinction coefficients (ε) were determined with standard deviation and errors based on the generated plot curves.

An Agilent (Melbourne, VIC, Australia) Technologies Cary 3500 UV–Vis Multicell Peltier spectrophotometer was utilised to perform the UV spectroscopy experiments. UV spectroscopic experiments were completed at room temperature in the range of 200–400 nm with a 1 cm quartz cuvette. All complexes were prepared in d.i.H₂O, while 5B3A was prepared in CH₃CN. A stock solution of each complex or compound (1 mM) was prepared, and absorption spectra were recorded at a series of different concentrations by titrating 9 $\times$ 3 µL aliquots into a cuvette containing d.i.H₂O (3000 µL). Experiments were repeated in triplicate. All spectra were baseline corrected by the instrument—a baseline containing d.i.H₂O was acquired first, and automatically subtracted from each experiment. Average extinction coefficients (ε) were determined with standard deviation and errors based on the generated plot curves.

An Agilent Technologies Cary 3500 UV-Vis Multicell Peltier spectrophotometer was utilized to perform the UV spectroscopy experiments. UV spectra were recorded at room temperature in the range of 200–400 nm with a 1 cm quartz cuvette. All complexes were prepared in d.i.H₂O. For the titration experiments, a stock solution of each complex (1 mM) was prepared and total aliquots of 9 $\times$ 3 µL were titrated into a cuvette containing d.i.H₂O (3000 µL). Experiments were repeated in triplicate. All spectra were baseline corrected and average extinction coefficients (ε) were determined with standard deviation and errors based on the generated plot curves.