Flow cytometric analysis was performed on a FACSymphony (BD Biosciences, Franklin Lakes, NJ). Antibodies used for analysis of NHP cells included CD34 PE-CF594 and CD34-APC clone 563 (BD Biosciences 561209 and 652449), CD90 PE clone 5E10 (BD Biosciences 555596), CD45 V450 clone D058-1283 (BD Biosciences 561291), and CD45RA APC-H7 clone 5H9 (BD Biosciences 561212). Dead cells and debris were excluded by forward/side scatter (FSC/SSC) gating.
Cd90 pe clone 5e10
Manufactured by BD
The CD90 PE clone 5E10 is a flow cytometry reagent used for the detection and analysis of CD90-positive cells. It consists of the CD90 monoclonal antibody conjugated to the fluorescent dye Phycoerythrin (PE). This reagent can be used to identify and characterize CD90-expressing cell populations in various samples.
Automatically generated - may contain errors
Lab products found in correlation
Cd45ra apc h7 clone 5h9, by BD (1 mentions)
Facsymphony, by BD (1 mentions)
Facscalibur cytometer, by BD (1 mentions)
Cd44 apc clone g44 26, by BD (1 mentions)
Lsr 2 cytometer, by BD (1 mentions)
Cd73 pe clone ad2, by BD (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
3 protocols using cd90 pe clone 5e10
1
Flow Cytometric Analysis of NHP Cells
2
MSC Marker Expression Analysis by FACS
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Expression of MSC markers was studied by fluorescence‐activated cell sorting. Cells were stained with CD90‐PE (clone 5E10; BD Pharmingen), CD44‐PE (clone MEM‐263; Abcam), CD29‐FITC (clone MEM‐101A; Abcam), swine leukocyte antigen SLA1‐FITC (clone JM1E3; AbD Serotec), CD105‐PE (clone MEM‐229; Abcam), and CD45‐FITC (clone 1E4; AbD Serotec) antibodies. FITC‐ or PE‐conjugated isotype antibodies (clone W3/25; AbD Serotec) were included as controls. Cell staining was analyzed on a Facscalibur Cytometer (Becton Dickinson).
3
Characterization of Cultured Mesenchymal Cells
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Cultured mesenchymal cells were incubated in PBS containing 3.3 mg/mL human immunoglobulin (Gammanorm; Octapharma) and 0.1% FBS (Gibco) for 10 min at 4 °C, to block unspecific binding to Fc-receptors, followed by staining with directly conjugated antibodies for 20 min at 4 °C. For cultured cells antibodies against the following surface markers were used: CD90-PE (clone 5E10), CD105-APC (clone 266), CD73-PE (clone AD2), CD44-APC (clone G44-26), CD166-BV421 (clone 3A6), CD10-BV421 (clone HI10a), CD13-PE (clone WM15), CD26-APC (clone M-A261) and CSPG4-PE (clone 9.2.27; all from BD Bioscience). Stained cells were run on a BD LSR II cytometer (BD Bioscience) and analyzed using FlowJo software version 10.5.3 (BD Bioscience).
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