The largest database of trusted experimental protocols
Sourced in United States

The AhR mAb is a monoclonal antibody that specifically binds to the Aryl hydrocarbon Receptor (AhR). AhR is a ligand-activated transcription factor that regulates the expression of genes involved in xenobiotic metabolism and other cellular processes.

Automatically generated - may contain errors

2 protocols using ahr mab

1

Profiling Xenobiotic Stress Responses

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cell pellets corresponded to untreated cells in media, 0.1% DMSO, B[a]P treated (1 μM), DATS treated (40 μM), and CoTx (1 μM B[a]P combined with 40 μM DATS) after 24 h treatment. 0.5% TritonX-100 was mixed with a protease inhibitor cocktail and combined with each pellet. The Pierce BCA Protein Assay kit was used to determine protein concentration. Each sample contained 50 μg of protein. 1:1000 dilution of primary antibody and secondary antibody were used. After the secondary antibody was incubated, the protein was identified, and the digital immunoblot was taken. The primary antibodies tested were CYP1A1 (ab235185) purchased from Abcam (Boston, MA, USA), Hypoxia Pathway Antibody Sampler Kit (#15792), AhR mAb (#83200), and loading control GAPDH mAb (#D16H11) purchased from Cell Signaling (Danvers, MA, USA).
+ Open protocol
+ Expand
2

Western Blot Analysis of Hypoxia Pathway

Check if the same lab product or an alternative is used in the 5 most similar protocols
The cell pellets were obtained from untreated cells in media alone, 0.1% DMSO vehicle control, B[a]P (1 μM), DATS (40 μM), and CoTx (1 μM B[a]P combined with 40 μM DATS), respectively, following a 24 h treatment. A mixture of 0.5% TritonX-100 and a protease inhibitor cocktail was added to each pellet, and the Pierce BCA Protein Assay kit was used to determine the protein concentration. Each sample possessed 50 μg of protein, and the primary and secondary antibodies were used at a dilution of 1:1000. Following the incubation with the secondary antibody, the protein was identified, and a digital immunoblot was captured. The primary antibodies assessed included CYP1A1 (ab235185) obtained from Abcam, the Hypoxia Pathway Antibody Sampler Kit (#15792), AhR mAb (#83200), and loading control GAPDH mAb (#D16H11) or Vinculin mAb (#13901) purchased from Cell Signaling.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!