Exosomes in osteoblast cell culture supernatant were isolated using an SBI ExoQuick-TC ULTRA EV Isolation Kit (System Biosciences, USA). Separation and reverse transcription of exosomes into cDNA were performed by using an SBI Seramir Exosome RNA Amplification Kit (System Biosciences, USA) and Cel-miR-39-3p standard RNA (RiboBio, Guangzhou, China) as external references. RT-PCR was performed to detect RNA expression in exosome using a FastStart Universal SYBR Green Master (ROX) kit (Roche, Germany).
Sbi exoquick tc ultra ev isolation kit
Manufactured by System Biosciences
Sourced in United States
The SBI ExoQuick-TC ULTRA EV Isolation Kit is a product designed for the isolation and purification of extracellular vesicles (EVs) from cell culture media or other liquid samples. The kit utilizes a proprietary polymer-based precipitation method to capture and concentrate EVs from the sample.
Automatically generated - may contain errors
2 protocols using sbi exoquick tc ultra ev isolation kit
1
Exosome Isolation and RNA Analysis
2
Exosome Isolation from Osteoblasts
Check if the same lab product or an alternative is used in the 5 most similar protocols
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SBI ExoQuick-TC ULTRA EV Isolation Kit (System Biosciences, USA) was used to isolate exosomes from osteoblasts culture supernatant. The isolated exosomes were resuspended in PBS, and 5 μL of the diluted mixture was dropped onto sealing films. Put the carbon-coated copper mesh on the droplets floating. Subsequently, the sealing film was stained with 20 μl of 1% phosphotungstic acid-staining droplet. After drying naturally, a standard TEM with a Philips CM120 microscope was used to observe exosomes and take a picture.
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