Supersignal west femto stable peroxide substrate

All herbs were purchased from Tong Ren Tang Chinese Medicine Co., Ltd. (Beijing, China), and carefully authenticated by Dr. P. F. Tu, Pharmacognosist, according to Chinese Pharmacopoeia (The Pharmacopoeia Commission of PRC, 2010). Aβ (1–42) were obtained from GL Biochem (Shanghai), Ltd. iNOS, p-IκB, IκB, p-IKKβ, IKKβ, p-NF-κB (p65), NF-κB, p-ERK, ERK, p-JNK, JNK, p-p38, p38, GAPDH, α-tubulin, Histone 3H polyclonal antibodies, and HRP conjugated anti-rabbit IgG were obtained from Cell Signaling Technology (Boston, MA, USA). SuperSignal West Femto Stable Peroxide substrate and AlexaFluor594-labeled goat anti-rabbit IgG antibody were obtained from Thermo Scientific (San Jose, CA, USA). 4′,6-Diamidino-2-phenylindole (DAPI) was purchased from Macgene (China).

Andrographolide (C₂₀H₃₀O₅) was purchased from Dalian Meilun Biotechnology Co., Ltd. (Dalian, China). The sample consisted of a white powder with a molecular weight of 350.45 and a purity of above 98% determined by HPLC analysis. OVA was purchased from Dalian Meilun Biotechnology Co., Ltd. (Dalian, China). The aluminum hydroxide gel was purchased from Thermo Fisher Scientific (Watham, MA, USA). The IL-6, IL-17A, and IL-17F ELISA kits were purchased from Multi Sciences (Hangzhou, China). Primary antibodies and HRP-conjugated anti-rabbit IgG were purchased from Bioworld Technology (Minnesota, USA), Cell Signaling Technology (Beverly, MA, USA), Affinity Bioscience (Shanghai, China), and Proteintech Group (Wuhan, China), respectively. SuperSignal West Femto Stable Peroxide Substrate and AlexaFluor594-labeled goat anti-rabbit IgG antibodies were obtained from Thermo Scientific (San Jose, CA, USA).