Oleanolic acid (OA) (99% purity) and rosmarinic acid (RA) (98% purity) were purchased from Sigma-Aldrich, and ursolic acid (UA) (92.84% purity) was purchased from the Resource Bank of Korean Medicine, National Institute for Korean Medicine Development. The compounds were dissolved in methanol at a concentration of 10 mM for use as controls, according to the methods described in previous studies (Qiang et al., 2011 (link); Chen et al., 2012 (link)). The PV extract was dissolved to 3.12 mg/ml, sonicated using ultrasound for 20 min, and filtered through a 0.45 μm syringe filter (Millipore Sigma, Burlington, MA, United States). HPLC analysis was conducted on an Agilent 1200 series system (Agilent Technologies, Santa Clara, CA, United States) equipped with a Shiseido UG120 column (5 μm, 6.4 × 250 mm) maintained at 25°C. The mobile phase was methanol (90%): 0.5% ammonium acetate (10%) for OA, UA, and methanol (52%), and 0.01% phosphoric acid (48%) for RA. Samples (20 μl) were analyzed at a flow rate of 0.6 ml/min using an ultraviolet detector at 210 nm for OA and UA, and at 310 nm for RA.
Ug120 column
Manufactured by Shiseido
Sourced in United States
The UG120 column is a laboratory equipment designed for liquid chromatography. It is used to separate and purify chemical compounds or biological samples based on their size or molecular weight. The column features a porous matrix that allows for efficient separation and high-resolution analysis. The specific details and intended uses of this product are not available.
Automatically generated - may contain errors
Lab products found in correlation
Ursolic acid, by Merck Group (1 mentions)
Rosmarinic acid, by Merck Group (1 mentions)
0.45 μm syringe filter, by Merck Group (1 mentions)
Oleanolic acid, by Merck Group (1 mentions)
Agilent 1200 series system, by Agilent Technologies (1 mentions)
1100 series hplc system, by Agilent Technologies (1 mentions)
2 protocols using ug120 column
1
Quantification of Bioactive Compounds in PV Extract
2
Extraction and Identification of S. baicalensis
Check if the same lab product or an alternative is used in the 5 most similar protocols
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S. baicalensis roots were obtained from Jung-do Herb, Co., Ltd. (Seoul, Korea). 50 g of dried S. baicalensis was extracted with 1 L boiling distilled water for 1 h 30 min. The extracts were filtered, concentrated and lyophilized. The dry weight of the S. baicalensis was 16.4 g (yield: 32.77% (w/w)). The voucher specimens were deposited at our laboratory. SB was identified by three standards, baicalin, baicalein and wogonin, using an Agilent Series 1100 HPLC system (Palo Alto, CA, USA) with a binary pump, an auto-sampler, a column oven, and a diode array detector (DAD). The Shiseido UG 120 column (250 × 4.6 mm, 5 μm) was tested with a guard column. The analysis was carried out at a flow rate of 1.0 mL/min with the detection wavelength at 280 nm. The HPLC peaks on SB were synchronized with baicalin, baicalein and wogonin (Fig. 1).
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