Protoblue safe colloidal coomassie g 250 stain

Commercial pea protein concentrate (Nutralys S85XF) (PPC) with 85% protein content was kindly gifted by Roquette (Lestrem, France). Sunflower oil was purchased from local supermarket and used without any further purification. Mini-PROTEAN TGX Gels, ProtoBlue Safe Colloidal Coomassie G-250 stain and all sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) chemicals were purchased from Bio-Rad Laboratories, UK. Sodium azide, Nile Red and Nile Blue were purchased from Sigma Aldrich (Dorset, UK). All other chemicals were of analytical grade and purchased from Sigma-Aldrich Dorset, UK) unless otherwise specified. All solutions were prepared with Milli-Q water (water purified by a Milli-Q apparatus, Millipore Corp., Bedford, MA, USA) with a resistivity of 18.2 MΩ cm at 25 ℃.

Pea protein concentrate (Nutralys S85X) containing 85% protein was provided by Roquette (Lestrem, France). Cellulose nanocrystal powder (CNC), which contained 100% sulfated CNC was purchased from CelluforceTM (Quebec, Canada). Sunflower oil was purchased from the local supermarket (Tesco, UK). Pepsin (P7000) with measured enzymatic activity of 650 U/mg was purchased from Sigma-Aldrich (Dorset, UK). Mini-protein TGX gels, ProtoBlue safe colloidal Coomassie G-250 stain and all chemicals for sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) were purchased from Bio-Rad Laboratories, UK. All the chemicals, including sodium azide, Nile Red and Nile Blue, were of analytical grade and purchased from Sigma-Aldrich (Dorset, UK) unless otherwise specified. All solutions used were prepared using Milli-Q water which was purified by a Milli-Q apparatus (Millipore Crop., Bedford, MA, USA) with an ionic purity of 18.2 MΩ•cm at 25℃.