Native tissue cell lysate kit

PLGA (MW: 90000, LA: GA = 50:50) was purchased from Jinan Daigang Bioengineering Co., Ltd. (Jinan, China); dichloromethane was purchased from Chengdu Kelong Chemical Co., Ltd. (Chengdu, China); UK and polyvinyl alcohol (PVA-210, MW: ~67,000) were purchased from Shanghai Aladdin Biochemical Technology Co., Ltd. (Shanghai, China); PFP was purchased from Strem Chemicals, Inc (Massachusetts, USA); Isopropanol was purchased from Chongqing Sichuan East Chemical Co., Ltd. (Chongqing, China); Bradford protein concentration assay kit, DAPI staining solution and 4% paraformaldehyde fix solution was purchased from Shanghai Biyuntian Biotechnology Co., Ltd. (Shanghai, China); DSPE-PEG-FITC, DSPE-PEG-RGD (the MW of PEG is 2000) were purchased from Hunan Huateng Pharmaceutical Co., Ltd. (Hunan, China); CCK-8 kit was purchased from Dojindo Institute of Chemistry (Japan); The native tissue/cell lysate kit was purchased from Beijing Solarbio Science & Technology Co.,Ltd. (Beijing, China); Dulbecco’s modified Eagle’s medium (DMEM), 1640 medium, fetal bovine serum (FBS), and penicillin-streptomycin were purchased from Gibco (USA).

The protein from different samples was extracted with native tissue/cell lysate kit (Solarbio, China). Then, the extracted protein samples were added to the loading wells of the SDS-PAGE gel, 20 μL per well. The gel was run at 75 V for 30 min, then the voltage was adjusted to 140 V until the protein band reached the bottom of the separating gel. Then, the gel was soak in Coomassie brilliant blue staining solution at room temperature for at least 4 h and the gel was decolorized by washing in Coomassie Brilliant Blue Destaining Solution until the bands were clear. Finally, the gel was imaged in a protein imaging system.