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Alkyne peg4 biotin

Manufactured by Thermo Fisher Scientific
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Alkyne-PEG4-biotin is a chemical compound used in various bioconjugation and labeling applications. It consists of an alkyne moiety, a polyethylene glycol (PEG4) linker, and a biotin functional group. This product can be used to facilitate the covalent attachment of biomolecules, such as proteins or nucleic acids, to surfaces or other molecules that contain azide or alkyne groups.

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Lab products found in correlation

Dimethyl sulfoxide (dmso), by Thermo Fisher Scientific (2 mentions) Dimethyl sulfoxide (dmso), by Merck Group (2 mentions) Mg132, by Merck Group (2 mentions) Dimethyl sulfoxide (dmso), by Santa Cruz Biotechnology (2 mentions) Chloroquine diphosphate salt, by Merck Group (2 mentions) L cystine dihydrochloride, by Merck Group (2 mentions) Bafilomycin a1, by Santa Cruz Biotechnology (2 mentions)

Spelling variants of this product

Biotin-alkyne (22 citations)

2 protocols using alkyne peg4 biotin

1

HCV NS3 Inhibitor Preparation and Utilization

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HCV NS3 inhibitors ciluprevir (CLV) and asunaprevir (ASV) were obtained by custom synthesis (Acme Bioscience) and dissolved in DMSO (Thermo) at 3 mM for medium-term storage at −20°C. These were then diluted into media to achieve the desired final concentration (1-3 μM) for treatment of cells. Further serial dilutions were performed for the dose-dependency experiment. For long-term CLV and ASV incubations, drug was applied simultaneously with transfection media or 1-2 h after transfection. MG132 (Sigma) was dissolved in DMSO for a 1000X working stock of 10 mM. Bortezomib (Adooq Biosci.) was dissolved in DMSO for a 500X working stock of 33 μM. Chloroquine diphosphate salt (Sigma) was dissolved in H2O for a 1000X (100 mM) working stock. Bafilomycin A1 (Santa Cruz) was dissolved in DMSO for a 500X (100 μM) working stock. Azidohomoalanine (AHA) (Click-IT, Invitrogen) was dissolved in DMSO for a 500X working stock of 25 mM. Alkyne-PEG4-biotin (Invitrogen) was dissolved in DMSO for a 100X working stock of 4 mM. L-cystine dihydrochloride (Sigma) was dissolved in H2O pH 2.0 for a working stock of 0.1 M.
Chung H.K., Jacobs C.L., Huo Y., Yang J., Krumm S.A., Plemper R.K., Tsien R.Y, & Lin M.Z. (2015). Tunable and reversible drug control of protein production via a self-excising degron. Nature chemical biology, 11(9), 713-720.
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2

HCV NS3 Inhibitor Preparation and Utilization

Check if the same lab product or an alternative is used in the 5 most similar protocols
HCV NS3 inhibitors ciluprevir (CLV) and asunaprevir (ASV) were obtained by custom synthesis (Acme Bioscience) and dissolved in DMSO (Thermo) at 3 mM for medium-term storage at −20°C. These were then diluted into media to achieve the desired final concentration (1-3 μM) for treatment of cells. Further serial dilutions were performed for the dose-dependency experiment. For long-term CLV and ASV incubations, drug was applied simultaneously with transfection media or 1-2 h after transfection. MG132 (Sigma) was dissolved in DMSO for a 1000X working stock of 10 mM. Bortezomib (Adooq Biosci.) was dissolved in DMSO for a 500X working stock of 33 μM. Chloroquine diphosphate salt (Sigma) was dissolved in H2O for a 1000X (100 mM) working stock. Bafilomycin A1 (Santa Cruz) was dissolved in DMSO for a 500X (100 μM) working stock. Azidohomoalanine (AHA) (Click-IT, Invitrogen) was dissolved in DMSO for a 500X working stock of 25 mM. Alkyne-PEG4-biotin (Invitrogen) was dissolved in DMSO for a 100X working stock of 4 mM. L-cystine dihydrochloride (Sigma) was dissolved in H2O pH 2.0 for a working stock of 0.1 M.
Chung H.K., Jacobs C.L., Huo Y., Yang J., Krumm S.A., Plemper R.K., Tsien R.Y, & Lin M.Z. (2015). Tunable and reversible drug control of protein production via a self-excising degron. Nature chemical biology, 11(9), 713-720.
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