System microscope bx63

Manufactured by Olympus

The System Microscope BX63 is a high-performance optical microscope designed for advanced research and clinical applications. It features a robust and ergonomic design, accommodating a range of objectives and accessories to support various imaging techniques.

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Lab products found in correlation

T7 ribomax kit, by Promega (1 mentions) Recombinant human ifn α2b, by ProSpec (1 mentions)

Close spelling variants of this product

BX63 microscope (334 citations) Olympus System Microscope (4 citations)

2 protocols using system microscope bx63

Tracking EV71-GFP Expression in SCARB2 Cells

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pWSK-EV71-GFP was linearized XbaI and EV71-GFP RNAs were transcribed using the T7 RiboMax kit (Promega). After transfection into 293A-SCARB2-L3HYPDH and 293A-SCARB2-Ctrl cells, the GFP signal was observed under a fluorescence microscope (System Microscope BX63, Olympus) at the indicated times; total RNAs were isolated for RT-qPCR assay.

Liu J., Liu L., Zeng S., Meng X., Lei N., Yang H., Li R., Mu X, & Guo X. (2024). Inhibition of EV71 replication by an interferon-stimulated gene product L3HYPDH. Virus Research, 342, 199336.

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IFN-α2b Inhibits EV-A71 Replication

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Different kinds of cells were treated with 1000 IU/ml of recombinant human IFN-α2b (Prospec) for the indicated time, and then total RNAs were isolated and used to measure specific mRNA abundance by RT-qPCR. To examine the inhibitory effect of IFN on EV-A71, Vero, 293A-SCARB2 and RD cells were infected with EV-A71-GFP and treated with IFN-α2b at 0, 1000 and 10000 IU/ml for 8 h. The replication of the virus EV-A71-GFP was estimated by observing the GFP signal under a fluorescence microscope (System Microscope BX63, Olympus).

Guo X., Zeng S., Ji X., Meng X., Lei N., Yang H, & Mu X. (2022). Type I Interferon-Induced TMEM106A Blocks Attachment of EV-A71 Virus by Interacting With the Membrane Protein SCARB2. Frontiers in Immunology, 13, 817835.

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