The following chemicals were used for the analysis of DON and its metabolites: 13C labelled DON standard (Sigma, Saint Louis, MO, USA; product number: 34128, 1.2 mL); DON (Sigma, Saint Louis, MO, USA; product number: D0156, 1 mg); β-glucuronidase (Type IX-A from E. coli; Sigma, Saint Louis, MO, USA; product number: G7396-2MU); DOM-1 (Sigma, Saint Louis, MO, USA; product number: 34135, 2 mL); and DONtest WBTM immunoaffinity columns (Vicam, Milford, MA, USA; product number: G1066). The equipment used was a Waters 2795 HPC Separation Module (Waters Corp., Milford, MA, USA) with a Quattro Micro Triple Quadrupole Mass Spectrometer (Micromass UK Ltd., Manchester, UK).
Waters 2795 hpc separation module
Manufactured by Waters Corporation
Sourced in United States
The Waters 2795 HPC Separation Module is a high-performance liquid chromatography (HPLC) instrument. It is designed for sample handling and separation of complex mixtures. The 2795 HPC Separation Module provides precise solvent delivery, automated sample injection, and temperature control to facilitate efficient chromatographic separations.
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2 protocols using waters 2795 hpc separation module
1
Analysis of DON and Metabolites
2
Quantitative Analysis of DON and Metabolites
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DON and its metabolites were analysed using 13 C labelled DON standard (Sigma, Saint Louis, MI, USA; product number: 34128, 1.2 mL), DON (Sigma, Saint Louis, MI, USA; product number: D0156, 1 mg), β-glucuronidase (Type IX-A from E. coli; Sigma, Saint Louis, MI, USA; product number: G7396 -2MU), DOM-1 (Sigma, Saint Louis, MI, USA; product number: 34135, 2 mL), and DON test WB TM immunoaffinity columns (Vicam, Milford, MA, USA; product number: G1066). All analyses were conducted on a Waters 2795 HPC Separation Module (Waters Corp., Milford, MA, USA) with a Quattro Micro Triple Quadrupole Mass Spectrometer (Micromass UK Ltd., Manchester, UK).
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