Ammonia test

Manufactured by Fujifilm

Sourced in Japan

The Ammonia test is a laboratory equipment used to measure the concentration of ammonia in a given sample. It provides a quantitative analysis of the ammonia level present, without interpretation or extrapolation of the intended use.

Automatically generated - may contain errors

Lab products found in correlation

Spelling variants of this product

Ammonia test kit (5 citations) Ammonia Test Wako (5 citations) Ammonia-Test-Wako kit (2 citations)

3 protocols using ammonia test

Ammonia Metabolism in Liver Buds

Check if the same lab product or an alternative is used in the 5 most similar protocols

Example 10

(Method)

Liver buds derived from PGECs (P15) were differentiated and induced, and 2 mM NH₄Cl was added to cell culture supernatant. Then, culture supernatant was collected at 0 hr, 3 hr, 6 hr and 24 hr, and ammonia concentration was measured with Ammonia Test (WAKO).

(Results)

It became clear that differentiated and induced PGEC-derived liver buds had a remarkable ammonia metabolizing function (FIG. 27).

US10370638B2. Primitive gut endoderm cells and method for producing same (2019-08-06). PUBLIC UNIVERSITY CORPORATION YOKOHAMA CITY UNIVERSITY [JP]. Inventors: Takanori Takebe [JP], Hideki Taniguchi [JP], Ran ran Zhang [JP].

+ Open protocol

+ Expand

Rumen Fluid Analysis Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

The rumen fluid was collected once during each collection period at 4 hr post‐feeding. Rumen fluid was aspirated using an oral probe. After filtering the ruminal fluid with four layers of gauze, it was frozen and preserved at −30°C until the concentration of volatile fatty acid (VFA), ammonium nitrogen (NH₃‐N), and lipopolysaccharide (LPS) level could be measured.
The VFA composition in the ruminal fluid was analyzed by high‐performance liquid chromatography (CTO‐10AV; Shimazu Corporation, Kyoto, Japan) using the bromothymol blue post‐label method. The concentration of ruminal NH₃‐N was quantified using an ammonia test (Wako Pure Chemical Industries, Ltd, Tokyo, Japan). The LPS level was measured in accordance with the method described by Hirabayashi et al. (2017).

Nishimura K., Kurosu K., Terada F., Mizuguchi H., Sato S, & Kushibiki S. (2018). Effect of wood kraft pulp feed on digestibility, ruminal characteristics, and milk production performance in lactating dairy cows. Animal Science Journal = Nihon Chikusan Gakkaiho, 90(2), 189-195.

+ Open protocol

+ Expand

Streptococcus thermophilus CO2 and NH3 Production

Check if the same lab product or an alternative is used in the 5 most similar protocols

Wild-type (WT) Strep. thermophilus and ∆ureC derivative cultures were used to inoculate at a final OD 660 of 0.05 in skim milk supplemented with 0.1% casein digested peptides and 0.4 g/L urea and, only for CO 2 measurement, flushed with N 2 gas for 30 min. These cultures were transferred to tightly closed glass bottles without headspace for CO 2 measurement or to plastic test tubes for NH 3 measurement and incubated at 43°C. After 6 h of cultivation, the CO 2 concentration of the medium was determined by a glass electrode method using a CO 2 concentration meter (CGP-31, DKK-TOA, Tokyo, Japan). For NH 4 -N measurement, sample and deproteinization agent (Wako Pure Chemical Industries. Ltd.) were mixed and centrifuged at 15,133 × g for 10 min at 4°C. The supernatant was filtered with a 0.22-µm cellulose acetate filter and used for NH 4 -N measurement by ammonia test (Wako Pure Chemical Industries Ltd.). The calibration curve using NH 4 Cl ranged from 1 to 8 mg/L NH 4 -N for WT and 1 to 4 mg/L NH 4 -N for ∆ureC.

Yamauchi R., Maguin E., Horiuchi H., Hosokawa M, & Sasaki Y. (2019). The critical role of urease in yogurt fermentation with various combinations of Streptococcus thermophilus and Lactobacillus delbrueckii ssp. bulgaricus. Journal of dairy science, 102(2).

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!