Theophylline

Kolliphor^® HS 15 (macrogol 15 hydroxystearate) was a gift from BASF (Ludwigshafen, ermany). Labrafac^® WL 1349 and Transcutol^® HP were kindly provided by Gattefossé S.A. (Saint-Priest, France). Tegaserod was purchased from Synthenova SAS (Hérouville-Saint-Clair, France). The peptide-22 was synthesized by the peptide team of the Institut des Biomolécules Max Mousseron (IBMM, Montpellier, France) according to Malcor et al. [39 (link)]. Acetylthiocholine iodide (ATC), butyrylthiocholine iodide and 5,5-dithiobis-(2-nitrobenzoic) acid (DTNB), human acetylcholinesterase (AChE), equine butyrylcholinesterase (BuChE), donepezil, Triton^® X-100 (octylphenol PEG-10 ether), KH₂PO₄, KCl and NaH₂PO₄·H₂O were purchased from Sigma-Aldrich (Saint-Quentin Fallavier, France). Tacrine was provided from Tocris (Lille, France). Prisma Buffer HT pH 7,4, sink brain buffer, corticosterone and theophylline was obtained from pION (Billerica, Etats-Unis, MA, USA). Methanol, acetonitrile, acetone, water of HPLC grade were purchased from Carlo Erba Reagents (Val de Reuil, France). Formic acid, Na₂HPO₄·12 H₂O, Tris hydrochloride, HEPES and DMSO were obtained from Fisher Scientific (Illkirch, France). NaCl was provided by Acros Organics (Geel, Belgium).

Soybean, and egg phosphatidylcholine (SPC and EPC, respectively) were gifts from Lipoid GmbH (Ludwigshafen, Germany). Cholesterol (CHOL), Sepharose^® CL-4B, and Chitosan oligosacharide lactate, donepezil, hydroxypropylmethyl cellulose, gellan gum, acetylthiocholine-iodide (ATC), butyrylthiocholine iodide and 5,5-dithiobis-(2-nitrobenzoic) acid (DTNB), human aceylcholinesterase (AChE), equine butyrylcholinesterase (BuChE), donepezil were purchased from Sigma-Aldrich (Ludwigshafen, Germany). Tacrine was provided from Tocris (Lille, France). Kolliphor® P407 (POE₁₀₁-POP₅₆-POE₁₀₁ triblock copolymer) and Kolliphor® P188 (POE₇₅-POP₃₀-POE₇₅ triblock copolymer) were gifts from BASF (Levallois-Perret, France). Sodium chloride was purchased from Carlo Erba (Val de Reuil, France). Prisma Buffer HT pH 7,4, sink brain buffer, corticosterone and theophylline was obtained from pION (Billerica, MA, Etats-Unis, USA). Methanol, acetonitrile, water of HPLC grade, formic acid, sodium hydroxide, and hydrochloric acid were provided by Prolabo VWR International (Fontenay-sous-Bois, France).