Pe mouse anti human cd2

Manufactured by BD

The PE mouse anti-human CD2 is a monoclonal antibody that binds to the CD2 antigen expressed on the surface of human T cells and natural killer cells. It is designed for use in flow cytometry applications to identify and quantify CD2-positive cell populations.

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2 protocols using pe mouse anti human cd2

Quantifying CSC and CXCR1 Populations

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CSC populations (CD24⁻/CD44⁺ and ALDH⁺) were measured by flow-cytometry of single-cell suspensions obtained from tumor biopsies (Additional file 1: Supplemental Materials) by using anti-human CD44-APC (clone G44-26) and CD24-FITC (clone ML5, RUO) antibodies (BD Biosciences, Franklin Lakes, NJ), and ALDEFLUOR assay (StemCell Technologies, Inc., Vancouver, BC, Canada), respectively, as previously published [13 (link)].
Additional analyses were directed to determine CXCR1 (PerCP/Cy5.5 mouse anti-human CD181 (CXCR1; BioLegend, San Diego, CA) and cell lineages using the following antibodies: APC mouse anti-human CD44 (BD Bioscience, San Jose, CA; catalog #559942), PE-Cy7 mouse anti-human CD24 (#561646), PE mouse anti-human CD2 (#555327), PE mouse anti-human CD31 (#555446), PE mouse anti-human CD3 (#555333), PE mouse anti-human CD18 (#555924), PE mouse anti-human CD16 (#555407), PE mouse anti-human CD19 (#555413), and PE mouse anti-human CD45 (#555483).

Goldstein L.J., Perez R.P., Yardley D., Han L.K., Reuben J.M., Gao H., McCanna S., Butler B., Ruffini P.A., Liu Y., Rosato R.R, & Chang J.C. (2020). A window-of-opportunity trial of the CXCR1/2 inhibitor reparixin in operable HER-2-negative breast cancer. Breast Cancer Research : BCR, 22, 4.

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Immunophenotyping of Transplant Recipients

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PBMCs were isolated from transplant recipients immediately prior to transplantation (POD 0) and immediately prior to every AT-1501 infusion outlined above. PBMCs were stained with the following antibodies: APC-Cy7 Mouse Anti-Human CD3 (BD Biosciences, clone SP34-2), PerCP-Cy5.5 Mouse Anti-Human CD4 (BD Biosciences, clone L200), PE Mouse Anti-Human CD2 (BD Biosciences, clone RPA-2.10), PE-Cy7 Mouse Anti-Human CD28 (Thermo Fisher Scientific, clone RPA-28.2), BV421 Mouse Anti-Human CD95 (BD Biosciences, clone DX2), APC-Cy7 Mouse Anti-Human CD20 (BD Biosciences, clone L27), PE Mouse Anti-Human CD56 (Thermo Fisher Scientific, clone TULY56), PE-Cy7 Mouse Anti-NHP CD45 (BD Biosciences, clone D058-1283), Alexa Fluor ® 488 anti-human FOXP3 (Biolegend, clone 206D), PE anti-human CD25, Anti-Human (Miltenyi Biotec, clone 4E3), PE-Cy7 CD127 (Thermo Fisher Scientific, clone eBioRDR5), FITC mouse anti-Ki-67 (BD Biosciences). T cell memory compartments were defined for analysis based on CD28 and CD95 expression following identification of CD3 + CD4 + or CD3 + CD8 + T cells. CD28 and CD95 gating to identify naïve (CD28 + CD95 -), central memory (CD28 + CD95 + ) and effector memory (CD28 -CD95 + ) was employed as previously described(68). Samples were acquired with an LSRFortessa flow cytometer (BD Biosciences) and data were analyzed with FlowJo software (version 10).

Anwar I.J., Berman D.M., DeLaura I., Gao Q., Willman M.A., Miller A., Gill A., Gill C., Perrin S., Ricordi C., Ruiz P., Song M., Ladowski J.M., Kirk A.D, & Kenyon N.S. (2023). The anti-CD40L monoclonal antibody AT-1501 promotes islet and kidney allograft survival and function in nonhuman primates. Science translational medicine, 15(711).

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