Three fragments of mouse PSD95 (dlg4) mRNA 3′UTR [3′UTR-A(2491-2769nt), 3′UTR-B(2770-3048nt) and 3′UTR-C(3049-3327nt)] were cloned into pMIR-REPORT luciferase plasmid (GeneChem, China). For luciferase reporter assays, HT-22 cells were co-transfected with pMIR-REPORT luciferase plasmid with PSD95 3′UTR-A, -B and -C (500 ng/well), Cirbp plasmids (500 ng/well) or NC (control) plasmids (500 ng/well), and Prl-TK plasmid (50 ng/well) using Lipofectamine 2000 (Invitrogen, USA). The luciferase activity was determined with the Dual-Luciferase Reporter Assay System according to the manufacturer’s instructions as described [53 ].
Pmir report luciferase plasmid
Manufactured by Genechem
Sourced in China
The PMIR-Report luciferase plasmid is a laboratory tool used to measure gene expression. It contains a luciferase reporter gene that can be used to quantify the activity of a promoter or regulatory sequence of interest. The plasmid can be transfected into cells, and the luciferase activity can be measured as an indicator of gene expression levels.
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Lab products found in correlation
2 protocols using pmir report luciferase plasmid
1
Examining PSD95 3'UTR Regulation
2
Cloning of mouse PSD95 3'UTR fragments
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Three fragments of mouse PSD95 (dlg4) mRNA 3'UTR [3'UTR-A(2491-2769nt), 3'UTR-B(2770-3048nt) and 3'UTR-C(3049-3327nt)] were cloned into pMIR-REPORT luciferase plasmid (GeneChem, China). For luciferase reporter assays, HT-22 cells were co-transfected with pMIR-REPORT luciferase plasmid with PSD95 3'UTR-A, -B and -C (500 ng/well), Cirbp plasmids (500 ng/well) or NC (control) plasmids (500 ng/well), and Prl-TK plasmid (50 ng/well) using Lipofectamine 2000 (Invitrogen, USA). The luciferase activity was determined with the Dual-Luciferase Reporter Assay System according to the manufacturer's instructions as described (46).
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