Floustar

Manufactured by BMG Labtech

Sourced in Germany

The Floustar is a fluorescence detection instrument designed for sensitive and accurate measurements in life science research applications. It provides a compact and flexible platform for a variety of fluorescence-based assays.

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Lab products found in correlation

Alamar blue, by BMG Labtech (1 mentions)

Close spelling variants of this product

FLOUstar Omega (8 citations) FLOUstar Optima (8 citations) FLOUstar Optima plate reader (5 citations) FLOUstar Omega microplate reader (3 citations) FLOUstar omega multiwell plate reader (2 citations)

3 protocols using floustar

Metal Oxide Nanoparticle Cytotoxicity Evaluation

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Vero E6 cells were cultured using Dulbecco’s
modified Eagle’s medium (DMEM) high glucose with 10% fetal
bovine serum (FBS) supplemented with penicillin–streptomycin, l-glutamine, and sodium pyruvate. Cells were seeded in a 96-well
plate and incubated for 24 h at 37 °C and 5% CO₂ for
attachment. The metal oxide nanoparticles (ZnO NPs and CuO NPs) of
calcined and noncalcined samples were autoclaved before suspension
in a sterile growth medium. Then serially diluted by concentrations
of 500, 250, 125, and 62.5 31.25 μg/mL, each sample was repeated
in triplicate. The cells were left for incubation with metal oxide
nanoparticles for 48 h. Thirty microliters of MTT (25 mg/mL) was added
to the cells and incubated for 3 h; then the medium was removed and
replaced with 100 μL of DMSO and left incubating for 10 min
before plate reading with Floustar (BMG labtech, Germany) at 570 nm.
The dose–response curve was used to calculate the IC₅₀.

Mahdy N.K., El-Sayed M., Al-Mofty S.E., Mohamed A., Karaly A.H., El-Naggar M.E., Nageh H., Sarhan W.A, & El-Said Azzazy H.M. (2022). Toward Scaling up the Production of Metal Oxide Nanoparticles for Application on Washable Antimicrobial Cotton Fabrics. ACS Omega, 7(43), 38942-38956.

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Quantifying Metabolic Activity via Alamar Blue

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Alamar Blue (alamarBlue®, Life Technologies, Grand Island, NY) was used to quantify differences in metabolic activity in response to exposure to TSH and oscillatory accelerations [32] (link). A 10x stock solution of Alamar Blue was diluted in calcium and magnesium free PBS to achieve a 1x final concentration. At the conclusion of each experiment, medium was removed, the wells were washed gently with PBS, and 100 µL of Alamar Blue solution was added to each well. Both the multi-well discs and controls were returned to the incubator for 1 h. The Alamar Blue solution was then transferred to a black 96-well plate and the fluorescence was measured (Ex/Em=560/590 nm) with a fluorometer (FLOUstar, BMG LABTECH Ltd., Ortenberg, Germany). Results are reported in arbitrary fluorescence units (AFU) with the blank value subtracted.

Wagner A.P., Chinnathambi S., Titze I.R, & Sander E.A. (2016). Vibratory stimulation enhances thyroid epithelial cell function. Biochemistry and Biophysics Reports, 8, 376-381.

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Biofilm Metabolic Activity Assay

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Biofilms were analyzed for metabolic activity using alamarBlue™ cell viability dye (Invitrogen, Inchinnan, UK). Once matured or after treatment and neutralization, alamarBlue™ was added at 10% well volume in RPMI/THB (e.g., 20 µL per 200 µL RPMI/THB) using a multichannel pipette in dark conditions. Plates were then incubated at 37 °C 5% CO₂, with a change from blue to pink indicative of a reduction in the fluorogenic dye resazurin to resorufin by enzymes involved in normal cellular respiration. The color change was read when the growth control reached a bright pink color to allow this to be used as a positive control or the latest after 3 h, depending on which occurred first. 100 µL from each well was transferred to a fresh 96 well flat bottom microtiter plate and top read using fluorescence 530/590 nm (FLOUstar™, BMG lab tech, Aylesbury, UK). A negative sterility control was also used on the same plate whereby no biofilm growth was initiated to use as a blank to normalize the fluorescence by an average of this value. In cases of treatment, a positive growth control (e.g., minus treatment) was included as a 100% growth value, to allow metabolic activity to be calculated as a percentage based on this growth control. All experiments assessing metabolic activity were completed using biofilms in triplicate or quadruplicate on three separate occasions.

Young T., Alshanta O.A., Kean R., Bradshaw D., Pratten J., Williams C., Woodall C., Ramage G, & Brown J.L. (2020). Candida albicans as an Essential “Keystone” Component within Polymicrobial Oral Biofilm Models?. Microorganisms, 9(1), 59.

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