On day 14 post-surgery mice of the SW 1990 and sham groups (SW 1990, n=5; sham, n=5) were anesthetized with isoflurane. An incision was made in the skin of the epigastric region and two silver wires were implanted in the rectus abdominis. The wires were implanted subcutaneously and exteriorized at the back of the neck. The mice were subsequently housed one per cage for 7 days.
Mice were placed individually in the center of an open field arena and the implanted wires were connected to a preamplifier (P5 Series; Grass Technologies, Warwick, RI, USA), electrical activity from the rectus abdominis was relayed to the Spike2 data acquisition program (version 7 for Windows; Cambridge Electronic Design, Cambridge, UK). The processed signal data designated VMRs were exported to Igor Pro software version 6.0 (WaveMetrics, Lake Oswego, OR, USA) for analysis (11 (link),12 (link)), and subtracted from background readings. To normalize VMRs, all VMRs from SW 1990 xenografted mice and sham-operated mice were divided by VMRs from normal mice. For all testing, the mice were habituated for 10 min, and then VMRs were recorded for 30 min. VMRs were recorded 5 times for each mouse, and the mean VMRs were calculated.
Mice were placed individually in the center of an open field arena and the implanted wires were connected to a preamplifier (P5 Series; Grass Technologies, Warwick, RI, USA), electrical activity from the rectus abdominis was relayed to the Spike2 data acquisition program (version 7 for Windows; Cambridge Electronic Design, Cambridge, UK). The processed signal data designated VMRs were exported to Igor Pro software version 6.0 (WaveMetrics, Lake Oswego, OR, USA) for analysis (11 (link),12 (link)), and subtracted from background readings. To normalize VMRs, all VMRs from SW 1990 xenografted mice and sham-operated mice were divided by VMRs from normal mice. For all testing, the mice were habituated for 10 min, and then VMRs were recorded for 30 min. VMRs were recorded 5 times for each mouse, and the mean VMRs were calculated.