Docosahexaenoic acid

Manufactured by Avanti Polar Lipids

Sourced in United States

Docosahexaenoic acid (DHA) is a long-chain polyunsaturated fatty acid. It is a major structural component of the human brain, cerebral cortex, skin, and retina.

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Lab products found in correlation

1 2 dioleoyl sn glycero 3 phosphocholine, by Avanti Polar Lipids (1 mentions) 1 2 dimyristoyl sn glycero 3 phosphocholine, by Avanti Polar Lipids (1 mentions) 1 2 dipalmitoyl sn glycero 3 phospho l serine, by Avanti Polar Lipids (1 mentions) Palmitic acid, by Avanti Polar Lipids (1 mentions) Stearic acid, by Avanti Polar Lipids (1 mentions) Oleic acid, by Avanti Polar Lipids (1 mentions) Linoleic acid, by Avanti Polar Lipids (1 mentions)

Close spelling variants of this product

Docosahexaenoic Acid-d5

2 protocols using docosahexaenoic acid

Lipid and Compound Preparation for Biophysical Studies

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1,2-Dimyristoyl-sn-glycero-3-phosphocholine
(DMPC), 1,2-dioleoyl-sn-glycero-3-phosphocholine
(DOPC), and 1,2-dipalmitoyl-sn-glycero-3-phospho-l-serine (DPPS, sodium salt) powders were purchased from Avanti
Polar Lipids (purity certified by the supplier >99%), whereas palmitic
acid (PA), stearic acid (SA), oleic acid (OA), elaidic acid (EA),
linoleic acid (LA), and docosahexaenoic acid (DHA), as well as nisin
(lyophilized powder containing ∼2.5% w/w nisin), solvents,
and the other chemicals were obtained from Sigma-Aldrich. The lipids
were of the highest available purity (≥99%) and were used without
further purification. All solvents were of analytical grade.

Saitta F., Motta P., Barbiroli A., Signorelli M., La Rosa C., Janaszewska A., Klajnert-Maculewicz B, & Fessas D. (2020). Influence of Free Fatty Acids on Lipid Membrane–Nisin Interaction. Langmuir, 36(45), 13535-13544.

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DHA Solubilization and Bioavailability Enhancement

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Docosahexaenoic acid (DHA) was obtained from Avanti Polar lipids (Alabaster, AL, USA) and dissolved in 100% ethanol at a concentration of 100 mM. DHA was conjugated to fatty acid-free bovine serum albumin (2% w/v) in media to increase both its solubility and bioavailability 56 . 25 μL of DHA stock or 25 μl of 100% ethanol (CON) was added per 50 mL of normal growth media containing 2% (w/v) of fatty acid-free bovine serum albumin serum (BSA; Sigma-Aldrich Pty Ltd, Sydney, NSW, Australia). Media was sterile filtered by passing it through a 0.2 μM PES filter, and then both CON and DHA-containing media were incubated in a water bath for 2 hours at 55ºC to conjugate DHA to BSA. Conjugated media was then stored at 4ºC until used. Cells were incubated overnight in the presence of CON or DHA-conjugated media prior to harvest.

Hancock S., Ding E., Beves E.J., Mitchell T.W., & Turner N. (2022). FACS-assisted single-cell lipidome analysis of phosphatidylcholines and sphingomyelins.

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