Htb 182

Human [A549, HCC-827 (adenocarcinomas), and HTB-182 (squamous cell carcinoma)] and a mouse (Lewis lung carcinoma) lung cancer cell lines were purchased from the American Type Culture Collection (ATCC). Cells were cultured in DMEM medium (Biological Industries, Beit Haemek, Israel) supplemented with 10% fetal calf serum (FCS) and antibiotics. Cells were infected with a lentivirus carrying the human heparanase cDNA, selected with blasticidin and pooled, as described [50 (link)]. Colony formation in soft agar and Matrigel invasion assay were performed essentially as described [50 (link), 51 (link)]. To quantify cellular invasion, the area covered by invading cells/filed was computed and presented graphically. Immunoblotting and ECM degradation heparanase assay were carried out as described [25 (link)–27 (link), 50 (link), 52 (link), 53 (link)].

Six NSCLC cell lines (HTB-182, A549, SPC-A-1, H1299, PC-9, LTEP-A-2) were obtained from the American Type Culture Collection. A normal human bronchial epithelial cell line (HBE), were purchased from the Institute of Biochemistry and Cell Biology of the Chinese Academy of Sciences (Shanghai, China). Cells were cultured in RPMI 1640 (GIBCO-BRL) medium supplemented with 10 % fetal bovine serum (10 % FBS), 100 U/ml penicillin, and 100 μg/ml streptomycin (Biyuntian, China) in humidified air at 37 °C with 5 % CO2.

Six NSCLC cell lines (HTB-182, A549, SPC-A-1, H1299, 95-D, Ltep-a-2) were obtained from the American Type Culture Collection. A normal human bronchial epithelial cell line (HBE), were purchased from the Institute of Biochemistry and Cell Biology of the Chinese Academy of Sciences (Shanghai, China). Cells were cultured in RPMI 1640 (GIBCO-BRL) medium supplemented with 10% fetal bovine serum (10% FBS), 100 U/ml penicillin, and 100 μg/ml streptomycin (Biyuntian, China) in humidified air at 37°C with 5% CO₂.