Serum levels of glucose, triglycerides, and non-esterified fatty acids were measured by enzymatic colorimetric methods (Wako Pure Chemical Industries). Serum insulin and adiponectin levels were tested using rat insulin (Morinaga Institute of Biological Science, Inc., Yokohama, Japan) and mouse/rat adiponectin (Otsuka Co., Tokyo, Japan) ELISA kits, respectively. Blood samples were collected, centrifuged at 1870×g for 15 min at 4 °C in a centrifuge (CF7D2; Hitachi Co. Ltd., Tokyo, Japan) and stored at − 80 °C until required for later analyses.
Cf7d2
Manufactured by Hitachi
Sourced in Japan
The CF7D2 is a laboratory centrifuge produced by Hitachi. It is designed to separate substances of different densities through the application of centrifugal force. The CF7D2 features a maximum speed of 7,000 rpm and can accommodate various rotor configurations to support different sample sizes and types.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using cf7d2
1
Serum Biomarkers Measurement Protocol
2
Serum Biomarker Measurement Protocol
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Blood was collected from the abdominal aorta under isoflurane anesthesia after the last urine collection. The tubes were standing for approximately 20 min at room temperature, and then they were transferred to on ice condition until centrifuging. The blood samples were centrifuged (rotor: RT3S3-A009; settings: 4°C, 3000 rpm, 15 min; CF7D2, Hitachi Koki Co., Ltd.) to separate serum, and the serum samples were used for the measurements of glucose, total protein, albumin, L-FABP, Cys-C and β2-MG because their urinary excretions were originated from blood. An enzymatic colorimetric method was used to measure the blood glucose, and a colorimetric method was used to measure the serum total protein and albumin (glucose, total protein, and albumin: Wako Pure Chemical Industries, Ltd.). All the measurements were made in a Hitachi 7180 biochemistry automatic analyzer (Hitachi High-Technologies Corporation). L-FABP, Cys-C, and β2-MG were measured using the same method as described above for the corresponding measurements in urine.
3
Schistosoma Soluble Egg Antigen Extraction
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Urine samples of S. haematobium- and/or S. mansoni-infected persons were centrifuged at 1500 rpm for 5 min, and supernatants were discarded. Pellets (containing schistosome eggs) were pooled and suspended in phosphate-buffered saline (PBS) and washed 3 times by centrifuging at low speed. Resulting pellet of eggs was then frozen at −80°C for 10 min. The frozen pellet was ultrasonified for 2 min in a repeated cycle of 5 times. When approximately 95% of the eggs were disrupted, the lysate was centrifuged for 20 min at 1000 rpm at 4°C (Hitachi CF7D2, Japan). The supernatant was then collected and ultracentrifuged for 90 min at 14,000 rpm at 4°C. The supernatant which was the Schistosoma spp soluble egg antigen (schSEA) was filter-sterilized and stored at −80°C until use [14 (link)]. Protein concentration of SEA was determined by the Bradford method [16 (link)].
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