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2 protocols using te 11

1

Culturing Human Esophageal Cancer Cell Lines

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The human esophageal cancer cell lines (Eca109, Eca9706, TE-1, TE-10, TE-11, KYSE140, and KYSE150) were obtained from the Type Culture Collection of Chinese Academy of Sciences (Shanghai, China) and cultured in RPMI-1640 medium (HyClone, USA) supplemented with 10% Fetal Bovine Serum (FBS) (Gibco-BRL), 100 μg/ml streptomycin and 100 units/ml penicillin G (Beyotime Biotechnology, Shanghai, China) in 5% CO2 and humidified atmosphere at 37 °C. Cells at the third generation of the exponentially growing state were used for all experiments.
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2

Culturing Human Esophageal Cancer Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
The human esophageal cancer cell lines (Eca109, Eca9706, TE-1, TE-10, TE-11, KYSE140, and KYSE150) were obtained from the Type Culture Collection of Chinese Academy of Sciences (Shanghai, China) and cultured in RPMI-1640 medium (HyClone, USA) supplemented with 10% Fetal Bovine Serum (FBS) (Gibco-BRL), 100 µg/ml streptomycin and 100 units/ml penicillin G (Beyotime Biotechnology, Shanghai, China) in 5% CO 2 and humidi ed atmosphere at 37℃. Cells at the third generation of the exponentially growing state were used for all experiments.
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