BV-2 and BC3H1 cells were incubated with tunicamycin (2 mg/ml) for 24 h. Following incubation, tumor cells were trypsinized and collected. Cells were then washed with ice-cold PBS, resuspended at 1×106 cells/ml in PBS and labeled with annexin V-fluorescein isothiocyanate (FITC) and propidium iodide (Annexin V-FITC kit; BD Biosciences) and analyzed using a FACScan flow cytometer (BD Biosciences). Apoptotic cells were analyzed using BD FACSDiva™ Software 1.2 (BD Biosciences).
Facsdiva software 1
Manufactured by BD
The BD FACSDiva™ Software 1.2 is a flow cytometry software application designed to control and analyze data from BD flow cytometers. It provides a user interface for configuring instrument settings, acquiring data, and processing acquired data.
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Lab products found in correlation
2 protocols using facsdiva software 1
1
Apoptosis Assay in Tumor Cells
2
Apoptosis Assay for TUN-treated Cells
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BV-2 and BC3H1 cells were incubated with TUN (2 mg/ml) for 24 h. Following incubation, the tumor cells were trypsinized and collected. The cells were then washed in cold PBS, adjusted to 1×106 cells/ml with PBS, labeled with Annexin V-fluorescein isothiocyanate (FITC) and propidium iodide (using the Annexin V-FITC kit; BD Biosciences), and analyzed with a FACScan flow cytometer (BD Biosciences) using BD FACSDiva™ Software 1.2 (BD Biosciences).
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