Heparin tube

This study was performed in the Department of Pharmacology and Toxicology, IL-YANG PHARM. Radotinib HCl and Nilotinib were suspended in 0.5% carboxymethyl cellulose at 50 mg/kg/10 ml, and were administered up to 6 weeks, female, ICR mice (Orient Bio. Gapyeong, Gyeonggi-do, Korea) by oral gavage. At 1, 2, 3, 4, 6, 8 and 24 h post dose, blood samples were collected by venipuncture of abdominal vena cava under anesthesia with Zoletil 50 (Virbac)/Rompun (Bayer). All blood samples were delivered into heparin tube (Fujifilm) and plasma was obtained by centrifugation at 3000 rpm, 15°C for 10 min. After blood sample collection, mice were perfused transcardially with 80 ml of PBS and whole brain was isolated. Whole brain was weighed and homogenized in PBS (1: 3 dilution). Then, 2-folds of acetonitrile were added to brain tissue sample and the mixture was centrifuged at 14 000 rpm, 4°C for 5 min. Supernatant was used for analysis. The concentration of Radotinib HCl and Nilotinib in plasma and brain tissue were measured using LC-MS/MS.

A portion of each fasting blood sample was immediately placed into blood collection tubes containing EDTA-2K and subjected to a complete blood count with a pocH-100 iV (Sysmex Corporation, Hyogo, Japan). The remaining blood was placed into a heparin tube (FUJIFILM Corporation, Tokyo, Japan) and centrifuged at room temperature at 7,700 rpm for 6 min. Using a DRI-CHEM 7000 V (FUJIFILM Corporation), we measured the following in the plasma obtained from centrifuging: blood urea nitrogen (BUN), Crn, total protein, albumin, total cholesterol, triglycerides, and blood glucose.