Mice were mated for 48 hours, and the presence of a vaginal plug was considered as gestational day 0. Pregnant mice received a single intraperitoneal (i.p.) injection of 600 mg/kg VPA (Sigma-Aldrich, China) or saline on gestational day 12.5. Only male offspring born from mothers exposed to saline or VPA were used. The male offspring were separated from the dams at 21st day of life and the rota rod test, three chamber sociability and social interaction test (3 CHM) were performed on postnatal day 54-55.
ceftriaxone (50–200 mg/kg, i.p.) was administered on postnatal days 47-55 for 8 days. ceftriaxone (Rocephin, Roche) was diluted from commercial preparations and dissolved in saline. Dihydrokainic acid 10 mg/kg (Sigma Aldrich, USA), a GLT-1 blocker, was co-administered with ceftriaxone 200 mg/kg group. All drugs and saline were administered i.p. in a volume of 0.1 mL/10 g body weight.
After having completed all behavioral tests, mice were euthanized and brain tissues were dissected for western blotting (WB) and immunohistochemistry (IHC) analysis.
ceftriaxone (50–200 mg/kg, i.p.) was administered on postnatal days 47-55 for 8 days. ceftriaxone (Rocephin, Roche) was diluted from commercial preparations and dissolved in saline. Dihydrokainic acid 10 mg/kg (Sigma Aldrich, USA), a GLT-1 blocker, was co-administered with ceftriaxone 200 mg/kg group. All drugs and saline were administered i.p. in a volume of 0.1 mL/10 g body weight.
After having completed all behavioral tests, mice were euthanized and brain tissues were dissected for western blotting (WB) and immunohistochemistry (IHC) analysis.