Eligibility criteria were age ≥18 years; microbiologically confirmed pulmonary TB by either smear positive for acid-fast bacilli (AFB), GeneXpert (Xpert MTB/RIF assay, Cepheid, Sunnywale, CA, USA), or AFB culture or clinical TB diagnosed using RNTCP clinical criteria; and known DM and HIV status [17 (link)]. Persons with prior TB history, rifampin-resistant TB, or multidrug-resistant TB, people with HIV (WH) infection, and pregnant women were excluded. INH monoresistance was not an exclusion criterion. Spot and early morning sputum specimens from individuals with possible TB in our concurrent prevalence study [17 (link)] underwent AFB, GeneXpert, and culture using Mycobacterial Growth Indicator Tube (MGIT, Becton Dickinson and Company, Sparks, MD, USA) liquid culture and Löwenstein-Jensen (LJ, EOS laboratories, Mumbai, Maharashtra , India) solid media methods. Baseline fasting or random blood glucose tests (Glucose HK, Roche Diagnostics GmbH, Mannheim, Gemany), HbA1c (Hemoglobin A1c, Bio-Rad Laboratories, Inc, Hercules, CA 94547, USA), and HIV (Determine HIV1/2, Alere Medical Co. Ltd. Chiba,270-2214, Japan) rapid tests were also performed. All microbiologic and blood-based tests were performed at the BJGMC-SGH laboratory.
Glucose hk
Manufactured by Roche
Sourced in United States, Germany
Glucose HK is a laboratory equipment product manufactured by Roche. It is designed for the quantitative determination of glucose in biological fluids. The core function of Glucose HK is to measure the concentration of glucose in samples using a hexokinase-based enzymatic method.
Automatically generated - may contain errors
3 protocols using glucose hk
1
Tuberculosis Diagnosis and Comorbidities
2
Biomarker Measurement Protocol for Metabolic Disorders
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IGFBP-1 was measured by ELISA (Beckman Coulter, Brea, CA) with an intra-assay precision of 2.5% and an inter-assay precision of 3.8%. RBP4 was measured by ELISA (ALPCO, Salem, NH). Glucose was measured by the hexokinase method (Glucose/HK; Roche Molecular Biochemicals, Werk Penzberg, Federal Republic of Germany). Plasma insulin was measured by solid-phase 125-I-RIA (Coat-A-Count; DPC, Los Angeles, CA). Total cholesterol, HDL-cholesterol, and triglycerides (TG) were determined by an autoanalyzer (Integra 400 Plus, Roche Diagnostics, Indianapolis, IN). LDL-Direct was calculated from total cholesterol, HDL, and TG using the Friedewald formula. Adiponectin was measured by RIA (Millipore, Billerica, MN). IL-6 and TNF-α were measured by ELISA (R&D Systems, Minneapolis, MN). CRP was measured by turbidimetric assay (Roche Diagnostics, Indianapolis, IN). The assays for IGFBP1 and TNF-α were performed in batches.
3
Fasting Lipid Profile and Glucose
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After 8 h of fasting, subjects' blood samples were taken and collected in ethylenediaminetetraacetic acid test tubes. Then, plasma and buffy coat were separated. Plasma was used to determine the lipid profile and fasting blood glucose levels and analyzed enzymatically with an automated analyzer (Cobas c111R analyzer with the protocol of Glucose HK, HDL-C Gen4, Triglycerides, Cholesterol Gen2 from Roche DiagnosticR, Germany). Buffy coat was used for DNA extraction using the FavorPrepᵀᴹ blood genomic DNA extraction mini Kit (Favorgen) with standard protocol and stored at -20°C.
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