Nanodrop one analyzer

Manufactured by Thermo Fisher Scientific

Sourced in United States

The Nanodrop One Analyzer is a compact, fixed-path spectrophotometer designed for the quantification and analysis of nucleic acids and proteins. It uses microvolume sample technology to accurately measure sample concentrations from 2 μL of liquid.

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Lab products found in correlation

Protease inhibitor, by Merck Group (1 mentions)

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NanoDrop (8392 citations) NanoDrop One (1486 citations) NanoDrop Analyzer (15 citations)

3 protocols using nanodrop one analyzer

Quantifying Photosynthetic Pigments in Seaweed

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The photosynthetic pigments (chlorophyll a—C_a, chlorophyll b—C_b, and carotenoids—C_x+c) were measured in an extract of 0.5 g of S. natans fresh weight (FW) in 5 mL of methanol. The plant–methanol mixture was vortexed thoroughly for 1 min and incubated at 70 °C for 3 min. The extracts were centrifuged, and the absorbance of the supernatant was measured at 665, 652, and 470 nm using a Nanodrop One Analyzer (Thermo Fisher Scientific, Waltham, MA, USA). The contents of the C_a, C_b, and C_x+c pigments were calculated according to Lichtenthaler (1987), and the results were expressed as µg/g (FW) [48 ].

Török A.I., Moldovan A., Kovacs E., Cadar O., Becze A., Levei E.A, & Neag E. (2022). Lithium Accumulation in Salvinia natans Free-Floating Aquatic Plant. Materials, 15(20), 7243.

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Protein Extraction and Quantification

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Samples were lysed using 300 μL of lysis buffer (10 mM 4-(2-hydroxyethyl-1-piperazineethanesulfonic acid) (HEPES), 42 mM potassium chloride, 0.1 mM ethylenediaminetetraacetic acid (EDTA), 0.1 mM ethylene glycol-bis(β-aminoethyl ether)-N,N,N’,N’-tetraacetic acid (EGTA), 1 mM dithiothreitol (DTT), 1x phosphatase inhibitor, and 1x protease inhibitor (Sigma-Aldrich)), homogenized, and sonicated. Further, 60 μL of 10% SDS was added to each sample and incubated for 10 minutes followed by centrifugation at 14,000 g for 45 minutes. The supernatant was collected, proteins were quantified using a NanoDrop One Analyzer (Thermo-Fisher), and samples were stored at −80°C.

Jones M.K., Lu B., Chen D.Z., Spivia W.R., Mercado A.T., Ljubimov A.V., Svendsen C.N., Van Eyk J.E, & Wang S. (2019). In vitro and in vivo proteomic comparison of human neural progenitor cell-induced photoreceptor survival. Proteomics, 19(3), e1800213.

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Quantifying Photosynthetic Pigments in S. natans

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The S. natans plants’ photosynthetic pigments (chlorophyll a—Chl a, chlorophyll b—Chl b, and carotenoids—Car (x + c)) were extracted using 0.5 g fresh weight (FW) plant biomass in 5 mL of methanol. The plant–methanol mixture was vortexed thoroughly for 1 min. and incubated at 70 °C for 3 min. The extracts were centrifuged, and the absorbance of the supernatant was measured at 665, 652, and 470 nm using a Nanodrop One Analyzer (Thermo Fisher Scientific, Waltham, MA, USA). The content of Chl a, Chl b, and Car (x + c) pigments was calculated according to Lichtenthaler (1987), and the results were expressed as µg/g (FW) [48 ].

Török A.I., Moldovan A., Senila L., Kovacs E., Resz M.A., Senila M., Cadar O., Tanaselia C, & Levei E.A. (2023). Impact of Low Lithium Concentrations on the Fatty Acids and Elemental Composition of Salvinia natans. Molecules, 28(14), 5347.

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