Asf 4 1

Human colorectal cancer cell lines DLD-1 and WiDr, human rhabdmyosarcoma cell line Rh30, human urinary bladder carcinoma cell lines T24 and 253J-BV, human stomach cancer cell lines KATOIII, NUGC3, and MKN7, and human prostate cancer cell line PC3 were maintained in RPMI-1640 medium (Wako Pure Chemical Industries, Ltd., Osaka, Japan), supplemented with 8% (v/v) heat-inactivated FBS (Sigma-Aldrich Co., St. Louis, MO, USA) and 2 mM l-glutamine. Human diploid fibroblast cell line ASF-4-1 and human rhabdmyosarcoma cell line RD were maintained in E-MEM medium (Wako), supplemented with 10% (v/v) heat-inactivated FBS and 2 mM l-glutamine. All cell lines were cultured under an atmosphere of 95% air and 5% CO₂ at 37 °C. DLD-1, WiDr, T24, 253J-BV, KATOIII, MKN7, RD, and ASF-4-1 cell lines were obtained from the JCRB cell bank (Osaka, Japan). The PC3 cell line was purchased from the American Type Cell Collection (ATCC). The Rh30 and NUGC3 cell lines were provided by Dr. Hosoi (Kyoto Prefectural University of Medicine) and Taiho Pharmaceutical (Tokyo, Japan), respectively. The number of viable cells was determined by performing the trypan-blue (Life Technologies, Carlsbad, CA, USA) dye exclusion test.