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3 protocols using 2 5 dimethylfuran

1

Synthesis of Organic Compounds

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DMDS (99%), glyoxylic acid (50% w/w), and 2-butanone (99%) were purchased from Alfa Aesar (Ward Hill, MA, USA). Anisole (>99%), 2-pentanone (>99%), MTA (>99%), 2-undecanone (>98%), and 2-nonanone (>98%) were purchased from Tokyo Chemical Industry (Tokyo, Japan). 2,5-dimethylfuran (99%) was purchased from Acros Organics (Geel, Antwerp, Belgium). Tween-20 was purchased from Sigma-Aldrich (St. Louis, MO, USA). Yeast extract and tryptone were purchased from Cyrusbioscience (Taipei, Taiwan) and Zymeset (Taipei, Taiwan), respectively. Potato extract was purchased from Formedium (Hunstanton, UK).
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2

Potato-based Microbial Media Preparation

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Potato infusion powder, peptone, tryptone, and yeast extract were purchased from DifcoTM (BD, Franklin Lakes, NJ, USA). Dimethyl disulfide was purchased from Alfa Aesar (Ward Hill, MA, USA), while 2,5-dimethylfuran was purchased from Acros Organics (Geel, Belgium). Other general chemicals were purchased from Sigma-Aldrich (St. Louis, MO, USA) and Merck (Darmstadt, Germany).
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3

Furan-based Chemicals Synthesis

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Nitric acid (70–71%, Aencore Chemical Co., LTD.), Urea for electrophoresis (99%, Sigma-Aldrich), i-propanol (HPLC grade, J.T. Baker), 5-hydroxymethylfurfural; 5-HMF (98%, AK scientific), 2,5-Bis (hydroxymethyl)furan; BHMF (98%, AK scientific); 2,5-dimethylfuran; 2,5-DMF (99%, Acros); 2,5-Dimethyltetrahydrofuran; DMTHF (mixture of cis- and trans-, 96%, Sigma-Aldrich); 5-Methylfurfural (5-MF, 99%, Sigma-Aldrich), 5-Methyl-2-furanmEthanol; MFA (97%, Acros), Ruthenium chloride n-Hydrate (39% metal content, UniRegion Bio-Tech), Hydrogen fluoride (HF, 48% Sigma-Aldrich), Hydrochloric acid ( ≥ 37%, Honeywell Fluka), Ethanol (99.9%, Honeywell Riedel-de-Haën), Aluminum (III) Chloride (98%, Sigma-Aldrich), Poly (ethylene glycol)-b-poly (propylene glycol)-b-poly (ethylene glycol), Pluronic P123 (Sigma-Aldrich), Furfuryl alcohol; FUL (98%, Acros Organics), Tetraethyl silicate (98%, Sigma-Aldrich), Ammonium hydroxide (28–30%, JT Baker Chemicals), Cetyltrimethylammonium bromide (98%, Alfa Aesar), Pluronic F-127(Sigma-Aldrich). Unless otherwise stated, all chemicals were used as received without further purification.
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