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3 5 dinitrosalicylic acid

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3,5-dinitrosalicylic acid is a chemical compound used in various laboratory applications. It is a yellow crystalline solid with a molecular formula of C7H5N2O5. The compound is commonly used as a reagent in colorimetric assays, particularly for the determination of reducing sugars.

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Lab products found in correlation

Gallic acid, by Merck Group (3 mentions) Methanol, by Merck Group (2 mentions) N hexane, by Merck Group (2 mentions) Ascorbic acid, by HiMedia (2 mentions) Maltopentaose, by Merck Group (1 mentions) Ethyl acetate, by Merck Group (1 mentions) 2 propanol, by Merck Group (1 mentions) Soluble starch, by HiMedia (1 mentions) Para nitrophenyl α d glucopyranoside, by Merck Group (1 mentions) α glucosidase, by HiMedia (1 mentions) Ascorbic acid, by Merck Group (1 mentions) Quercetin, by Merck Group (1 mentions) 2 4 dinitrophenyl hydrazine, by HiMedia (1 mentions) Calcium chloride, by HiMedia (1 mentions) Heptadecanoic acid, by Merck Group (1 mentions) Methyl orange indicator, by HiMedia (1 mentions) 2 2 azino bis 3 ethylbenzothiazoline 6 sulfonic acid, by Merck Group (1 mentions) Trichloroacetic acid tca, by Sisco Research Laboratories (1 mentions) Rutin, by Merck Group (1 mentions) Ferric chloride, by Sisco Research Laboratories (1 mentions)

5 protocols using 3 5 dinitrosalicylic acid

1

Carbohydrate Characterization Protocol

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Maltopentaose and HPA were purchased from Sigma Aldrich. Soluble
starch was procured from SRL, Mumbai, India. d-Maltose and
3,5-dinitrosalicylic acid (DNSA) were obtained from HiMedia Laboratories,
Mumbai, India. Solvents like methanol, 2-propanol, n-hexane, and ethyl acetate were obtained from Merck India Ltd., Mumbai.
Chemicals and solvents were of AR and HPLC grades.
Dandekar P.D., Kotmale A.S., Chavan S.R., Kadlag P.P., Sawant S.V., Dhavale D.D, & RaviKumar A. (2021). Insights into the Inhibition Mechanism of Human Pancreatic α-Amylase, a Type 2 Diabetes Target, by Dehydrodieugenol B Isolated from Ocimum tenuiflorum. ACS Omega, 6(3), 1780-1786.
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2

Enzymatic Starch Hydrolysis Protocol

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Potassium dihydrogen phosphate, sodium hydroxide, hydrochloric acid, agar, calcium chloride dihydrate (Qualigens Fine Chemicals, Mumbai, India), and soluble starch and 3,5-dinitrosalicylic acid (DNS) (Himedia Laboratories Pvt. Ltd., Mumbai, India) were used as received. Fungal α-amylase (source Aspergillus oryzae), sodium potassium tartrate, coomassie brilliant blue G, and ortho-phosphoric acid were purchased from S. D. Fine-Chem Ltd., Mumbai, India. All other chemicals and solvents were of analytical grade and were used without further purification. Double-distilled water was used throughout the study.
Sharma M., Sharma V, & Majumdar D.K. (2014). Entrapment of α-Amylase in Agar Beads for Biocatalysis of Macromolecular Substrate. International Scholarly Research Notices, 2014, 936129.
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3

Enzymatic Evaluation of Amylase and Glucosidase

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Chemicals such as soluble starch and porcine pancreatic α-amylase (PPA) were purchased from SRL Pvt. Ltd. (Mumbai, India). α-Glucosidase, 3,5-dinitrosalicylicacid (DNSA), and 2,4-dinitrophenyl hydrazine (DNPH) were obtained from HiMedia Laboratories (Mumbai, India). Para-nitrophenyl α-d-glucopyranoside and standards such as gallic acid, quercetin, and ascorbic acid were purchased from Sigma-Aldrich Co. (St. Louis, MO, USA). Acarbose was purchased from Bayer Scientific (Leverkusen, Germany). All other reagents and chemicals were of analytical grade and procured locally in Chennai.
Duraiswamy A., Shanmugasundaram D., Sasikumar C.S., Cherian S.M, & Cherian K.M. (2015). Development of an antidiabetic formulation (ADJ6) and its inhibitory activity against α-amylase and α-glucosidase. Journal of Traditional and Complementary Medicine, 6(3), 204-208.
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4

Comprehensive Chemical Reagents for Analytical Procedures

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Various chemicals of analytical
grade like hydrochloric acid (HCl), sulfuric acid (H2SO4), nitric acid (HNO3), phosphoric acid (H3PO4), sodium tungstate (Na2WO4·2H2O), sodium carbonate (Na2CO3), ammonium
molybdate ((NH4)6Mo7O24), ferric chloride (FeCl3), sodium chloride (NaCl), potassium
ferricyanide (K3Fe(CN)6), potassium permanganate
(KMnO4), sodium phosphate buffer, sulfosalicylic acid,
trichloroacetic acid (TCA), and potassium persulfate (K2S2O8) were obtained from Sisco Research Laboratory
(Mumbai, India). Liquified phenol, 2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic
acid (ABTS), boron trifluoride-methanol (BF3-methanol),
tannic acid, gallic acid, rutin, aluminum chloride (AlCl3), and heptadecanoic acid were purchased from Sigma-Aldrich (Bangalore,
India). 2,2-Diphenyl-picrylhydrazyl (DPPH), 3,5-dinitrosalicylic acid,
bovine serum albumin, methyl orange indicator, calcium chloride, phytic
acid, folin-Ciocalteau’s reagent, and ascorbic acid were obtained
from Hi-Media Labs, Mumbai, India. Solvents like acetone, n-hexane,
methanol, and absolute ethanol were procured from Merck (Mumbai, India).
Riya P., Kumar S.S, & Giridhar P. (2023). Phytoconstituents, GC-MS Characterization of Omega Fatty Acids, and Antioxidant Potential of Less-Known Plant Rivina humilis L.. ACS Omega, 8(31), 28519-28530.
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5

Antioxidant and Antidiabetic Assays

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Folin-Ciocalteu’s reagent, sodium carbonate, aluminium chloride, potassium acetate, quercetin, Mueller-Hilton agar medium, DPPH (2,2-diphenyl-1-picrylhydrazyl), ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)), potassium persulfate, ammonium molybdate, trisodium phosphate, potassium ferrocyanide, glucose, hemoglobin, sodium azide, 3, 5-dinitrosalicylic acid, ascorbic acid and α-amylase were purchased from Himedia (Bangalore, India). α- glucosidase, p-NPG (p-nitro phenyl α-d-glucopyranoside)), gallic acid, acarbose was purchased from Sigma–Aldrich (Bangalore, India). Standard anti-microbial drug, gentamycin and antidiabetic drug, metformin were purchased from the local market. All other chemical reagents and buffers were used as an analytical grade in this study.
Yarrappagaari S., Gutha R., Narayanaswamy L., Thopireddy L., Benne L., Mohiyuddin S.S., Vijayakumar V, & Saddala R.R. (2020). Eco-friendly synthesis of silver nanoparticles from the whole plant of Cleome viscosa and evaluation of their characterization, antibacterial, antioxidant and antidiabetic properties. Saudi Journal of Biological Sciences, 27(12), 3601-3614.
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