Hplc grade acetonitrile

APAP and resveratrol (RES) were purchased from Shanghai Source Leaf Biological Technology Co., Ltd. HPLC-grade acetonitrile, methanol and formic acid were obtained from Dikma Technologies Inc. (Beijing, China). Deionized water (18.2 MΩ) was obtained from a Milli-Q water purification system (Millipore, Bedford, MA, USA). Assay design enzyme-linked immunosorbent assay kit (ELISA) was obtained from Nanjing Jiancheng Bioengineering Institute. All the reference standards were obtained from Chengdu Mansite Bio-technology Co., Ltd. The Zhishi sample was collected from Jiangxi province of China, and its species, local name, collection location, year of collection and growing environment are listed in the complementary materials. Its dried specimens were deposited at the Institute of Basic Theory, China Academy of Chinese Medical Sciences, Beijing, P. R. China.

Reference standards of myricitrin, isoquercitrin, quercitrin, amentoflavone and hinokiflavone were purchased from Chengdu Desite Bio-Technology Co., Ltd., (Chengdu, China). PSA (40–60 μm, 60 A), NH₂ (40–60 μm, 60 A), CN (50 μm, 60 A), COOH (50 μm, 60 A), Florisil (60–100 μm, 80 A), C₈ (50 μm, 60 A), C₁₈ (50 μm, 60 A), C₁₈-N (50 μm, 60 A) and AQ C₁₈ (50 μm, 60 A) were supplied from Agela Technologies. 1-butyl-3-methylimidazolium tetrafluoroborate ([Bmim]BF₄), 1-hexyl-3-methylimidazolium tetrafluoroborate ([Hmim]BF₄), 1-octyl-3-methylimidazolium tetrafluoroborate ([Omim]BF₄), 1-butyl-3-methylimidazolium hexafluorophosphate ([Bmim] PF₆) and 1-octyl-3-methylimidazolium hexafluorophosphate ([Omim]PF₆) were purchased from Shanghai Chengjie Chemical Co., Ltd., HPLC-grade acetonitrile and methanol were purchased from Dikma Technologies Inc., United States. HPLC-grade formic acid was purchased from Tedia Company, Inc. (Tedia, Fairfield, OH, United States). Deionized water was purified from a Milli-Q academic ultra-pure water system (Millipore, Milford, MA, United States). Other chemical reagents were of analytical grade. All the solutions were filtered through a 0.22 μm filter membrane before UPLC analysis.

The Taq PCR Master Mix, DNA Marker DL2000 were acquired from TransGen Biotech (Beijing, China). Sodium hypochlorite solution, dextrose, agar were purchased from BBI Life Sciences Corporation. FB standards (FB₁ and FB₂) were obtained from Sigma Chemical Co. (St. Louis, MO, USA). HPLC-grade acetonitrile and methanol used for sample preparation and mobile phase were supplied by Dikma (Lake Forest, CA, USA). 2-Mercaptoethanol, OPA, and potassium borate buffer o-phthalaldehyde diluent (OD104) were purchased from Pickering Laboratories (Mountain View, CA, USA). Ultrapure water was prepared in all analytical steps using a Barnstead LabTower EDI water purification system (ThermoFisher Scientific, Waltham, MA, USA).