Mouse monoclonal anti phosphoerk1 2

All primary and secondary antibodies were diluted in PBS Tween-20 0.1% containing BSA 2%. Primary antibodies used were: rabbit polyclonal anti-HADHA (Proteintech, Cat# 10758-1-ap) 1:500; rabbit polyclonal anti-SQSTM1/p62 (Novus, Cat# NBP-48320, Centennial, CO, USA) 1:4000; mouse monoclonal anti-phosphoERK1/2 (Santa Cruz, Cat# 7383, Dallas, TX, USA) 1:500; rabbit polyclonal anti-ERK1 and anti-ERK2 (Santa Cruz, Cat# sc-93 and Cat# sc-154) 1:500; rabbit polyclonal anti-p21 (Proteintech, Cat# 10355-1-AP) 1:800; rabbit polyclonal anti-LC3 I/II (Novus, Cat# NB100-2220) 1:1000; rabbit monoclonal anti-phospho4EBP1 (Cell Signaling Technology, Cat# 2855T, Danvers, MA, USA) 1:500; mouse monoclonal anti-4EBP1 (Proteintech, Cat# 60246-1-1g) 1:500; mouse monoclonal anti-β Actina (Sigma Aldrich, Cat# A5441) 1:10,000.
Goat polyclonal anti-mouse 1:15,000 (Santa Cruz, Cat# sc-2005) and anti-rabbit 1:15,000 (Santa Cruz, Cat# sc-2004) were used as secondary antibodies.

Apigenin (HPLC>98%) was purchased from the Nanjing TCM Institute of Chinese Materia Medica and was dissolved in sodium carbonate (20 mM). Purified mouse anti-Bax and anti-Bcl-2 antibodies were purchased from Biolegend (USA). Mouse monoclonal anti-phospho-ERK1/2, anti-caspase-3, anti-caspase-8, anti-phospho-p38, rabbit monoclonal anti-ERK1/2, anti-p38, mouse monoclonal anti-β-actin, rabbit/goat anti-mouse IgG and goat anti-rabbit IgG antibodies were purchased from Santa Cruz Biotechnology (USA). Mouse monoclonal anti-phospho-JNK and anti-JNK antibodies were purchased from Cell Signaling Technology (USA). Apoptosis, DNA Ladder Extraction Kit with Spin Column, DNA Molecular Weight Marker was purchased from Beyotime Institute of Biotechnology (Shanghai, China). In Situ Cell Death Kit, Fluorescein was purchased from Roche Applied Science, Mannheim, Germany.