The largest database of trusted experimental protocols

3 protocols using sephadex g25 gel

1

Nanoparticle Synthesis and Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols
We purchased Distearoyl-sn-glycero-3-phosphocholine (DSPC) from Avanti Polar Lipids (Alabaster, AL), Cholesterol from Sigma-Aldrich (St. Louis, MO) and syringe filters from Corning (Lowell, MA). The Sephadex G-25 gel used in the study was purchased from Sigma-Aldrich (St. Louis, MO). We bought the anti-CD31 FITC antibody from Thermo Fisher Scientific (Rockford, IL), the tissue-freezing medium from the Molecular Cytology Core Facility (University of Missouri, Columbia, MO), the EtBRIII (necrosis study) from Enzo Life Sciences (Farmingdale, NY). Lastly, the EMT-6 cells from ATCC (Manassas, VA).
+ Open protocol
+ Expand
2

Cyclodextrin-Mediated Drug Formulation

Check if the same lab product or an alternative is used in the 5 most similar protocols
α-CD, β-CD, γ-CD, and randomly methylated-β-CD (RAMEB, DS = 12.9), were provided by Wacker Chemie (Germany). Low methylated-β-CD (CRYSMEB, DS = 4.9) and hydroxypropyl-β-CD (HPBCD, DS = 5.6) were provided by Roquette Frères (Lestrem, France). Sulfobutyl ether-β-CD (SBE-β-CD, DS = 6.5) was provided by LIGAND Pharmaceuticals (San Diego, CA, USA). Dipalmitoylphosphatidylcholine (DPPC) and trizma base (buffer reagent) were purchased from Sigma-Aldrich, Switzerland. Triton X-100, sodium chloride (NaCl), and Sephadex G25 gel were purchased from Sigma-Aldrich, Belgium. Ammonium molybdate, hydrogen peroxide, potassium dihydrogen phosphate, sodium sulfite, sodium bisulfite, chloroform, and methanol were purchased from Sigma-Aldrich, Germany. Cholesterol and sulforhodamine B were purchased from Sigma-Aldrich, USA. 4-Amino-3-hydroxy-1-naphthalene sulfonic acid was purchased from Fluka, India. Sulfuric acid was purchased from ACROS Organics, Belgium and diethyl ether was purchased from VWR-Prolabo Chemicals, Belgium.
+ Open protocol
+ Expand
3

Liposome Preparation and Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols
Dipalmitoylphosphatidylcholine (DPPC) (≥ 99%) and trizma base (buffer reagent) were purchased from Sigma-Aldrich, Swiss. Triton X-100, sodium chloride (NaCl) and Sephadex G25 gel were purchased from Sigma-Aldrich, Belgium. Sodium azide, ammonium molybdate, hydrogen peroxide, potassium dihydrogenophosphate, sodium sulfite, sodium bisulfite, menthol, eugenol, chloroform and methanol were purchased from Sigma-Aldrich, Germany. Linalool was purchased from Sigma-Aldrich, Switzerland. Trans-Anethole was purchased from Sigma-Aldrich, Spain. Geraniol and nerolidol were purchased from Sigma-Aldrich, USA. Sulforhodamin B and camphor were purchased from Sigma-Aldrich, China. Borneol, bornyl acetate, p-cymene, ɑterpinene and ɣ-terpinene were purchased from Fluka Chemical, Switzerland. 4-amino-3hydroxyl-1-naphtalene sulfonic acid was purchased from Fluka, India. Anisole was purchased from Fluka, Germany. Guaiacol, thymol, estragole, isoeugenol were purchased from ACROS Organics, New Jersey, USA. The sulfuric acid was purchased from ACROS Organics, Belgium.
The diethylether was purchased from VWR-Prolabo Chemicals, Belgium. β-caryophyllene and eugenyl acetate were purchased from SAFC, USA. Linalyl acetate was purchased from SAFC, Spain. ɑ-Phellandrene was purchased from SAFC, Germany. Camphene was purchased from MERCK, Schurchardt.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!