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Bax and bcl 2 human simplestep elisa kits

Manufactured by Abcam
Sourced in United Kingdom

The Bax and Bcl-2 Human SimpleStep ELISA® Kits are quantitative sandwich enzyme-linked immunosorbent assays (ELISAs) designed to measure the levels of Bax and Bcl-2 proteins in human cell and tissue samples. The kits utilize an antibody specific to the target proteins that is pre-coated onto a microplate.

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3 protocols using bax and bcl 2 human simplestep elisa kits

1

Apoptosis Induction Assay Protocol

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RPMI-1640 medium, Mycoplex™ fetal bovine serum (FBS), penicillin and streptomycin (100×), Dulbecco's Modified Eagle Medium (DMEM), and trypsin-ethylenediaminetetraacetic acid (EDTA) (1×) were bought from Gibco (Grand Island, NY, USA). Cycle TEST PLUS DNA Reagent Kit and Annexin V-FITC Apoptosis Detection Kit I were procured from BD Biosciences Pharmingen (Franklin Lakes, NJ, USA). Mitochondrial Membrane Potential Assay Kit (orange fluorescence) was bought from Abnova (Taipei City, Taiwan). Bax and Bcl-2 Human SimpleStep ELISA® Kits were obtained from Abcam, UK. Caspase Colorimetric Assay Kit was bought from R&D Systems (Minneapolis, MN, USA). All other reagents and chemicals used were of analytical grade and obtained from Sigma-Aldrich (St. Louis, MO, USA).
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2

Phytic Acid-Chitosan Interactions in Cell Assays

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Phytic acid sodium salt from rice (≥90% purity) and chitosan (deacetylated at 75–85%) were bought from Sigma-Aldrich (St. Louis, MO, USA). Trypsin EDTA (1×), Dulbecco′s Modified Eagle Medium (DMEM), RPMI-1640 medium, Mycoplex™ fetal bovine serum (FBS), and penicillin and streptomycin (100×) were bought from Gibco (Grand Island, NY, USA). Bax and Bcl-2 Human SimpleStep Elisa® Kits were procured from Abcam (Cambridge, UK).
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3

Apoptosis analysis of cell lines

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Mycoplex™ fetal bovine serum (FBS), trypsin EDTA (1×), penicillin and streptomycin (100×), and RPMI-1640 medium were bought from Gibco (Grand Island, NY, USA). Annexin V-FITC Apoptosis Detection Kit I and Cycletest Plus DNA Reagent Kit were bought from BD Biosciences Pharmingen (Franklin Lakes, NJ, USA). Caspase Colorimetric assay kit was purchased from R&D Systems (Minneapolis, MN, USA). Bax and Bcl-2 Human SimpleStep ELISA® Kits were procured from Abcam, UK.
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